ITMO University, SCAMT laboratory 9, Lomonosova str., Saint Petersburg, 191002, Russian Federation.
Sci Rep. 2019 Feb 4;9(1):1176. doi: 10.1038/s41598-018-37589-1.
Alumina is one of the most promising carriers for drug delivery due to the long history of its usage as a vaccine adjuvant. Sol-gel synthesis provides excellent conditions for entrapment of biomolecules within an inorganic cage providing stabilization of proteins under the extremal conditions. In this paper, we show in vitro investigation of monodisperse alumina xerogel nanocontainers (AXNCs) using bovine serum albumin as a model protein entrapped in sol-gel alumina building blocks. Particularly, dose and cell-type dependent cytotoxicity in HeLa and A549 cancer cell lines were employed as well as investigation of antibacterial effect and stability of AXNCs in different biological media. It was shown, that the release of entrapped protein could be provided only in low pH buffer (as in cancer cell cytoplasm). This property could be applied for anticancer drug development. We also discovered boehmite nanoparticles effect on horizontal gene transfer and observed the appearance of antibiotic resistance by means of exchanging of the corresponding plasmid between two different E. coli strains. The present work may help to understand better the influence of AXNCs on various biological systems, such as prokaryotic and eukaryotic cells, and the activity of AXNCs in different biological media.
氧化铝由于其作为疫苗佐剂的悠久历史，是最有前途的药物输送载体之一。溶胶-凝胶合成为将生物分子包埋在无机笼中提供了极好的条件，在极端条件下稳定蛋白质。在本文中，我们使用牛血清白蛋白作为模型蛋白，研究了溶胶-凝胶氧化铝砌块中包埋的单分散氧化铝干凝胶纳米容器（AXNCs）的体外研究。特别是，我们研究了 AXNCs 在 HeLa 和 A549 癌细胞系中的剂量和细胞类型依赖性细胞毒性，以及在不同生物介质中的抗菌效果和稳定性。结果表明，只有在低 pH 缓冲液（如癌细胞质中）才能提供包埋蛋白的释放。这种特性可用于抗癌药物的开发。我们还发现了拟薄水铝石纳米颗粒对水平基因转移的影响，并通过在两种不同的大肠杆菌菌株之间交换相应的质粒来观察抗生素抗性的出现。本工作有助于更好地了解 AXNCs 对各种生物系统（如原核细胞和真核细胞）的影响，以及 AXNCs 在不同生物介质中的活性。
