Hatami Mehrnaz, Karimi Mahnaz, Aghaee Ahmad, Bovand Fatemeh, Ghorbanpour Mansour
Department of Medicinal Plants, Faculty of Agriculture and Natural Resources Arak University Arak Iran.
Department of Horticultural Sciences Sari Agricultural Sciences and Natural Resources University Sari Iran.
Food Sci Nutr. 2022 Jul 22;10(11):3905-3919. doi: 10.1002/fsn3.2987. eCollection 2022 Nov.
Eryngo ( Trautv) a widespread species of the Apiaceae reveals high nutritional value and therapeutic properties due to the significant content of biologically active metabolites such as essential oils, phenolic compounds, and flavonoids. The present study was performed to evaluate the morphological and biochemical variability and antioxidant properties of naturally grown populations of eryngo. One-way ANOVA showed significant ( < .01) differences in the majority of parameters measured among the studied populations. The range of fresh weight was from 1.3 to 12.0 g/plant, while dry weight varied from 0.01 to 6.0 g/plant. The highest variation was observed for essential oil yield (CV = 205.32%) followed by essential oil content (CV = 126.23%) and chicoric acid content (CV = 71.18%). Total phenolics content varied from 8.85 to 88.15 mg GAE/g extract. Total flavonoids value ranged from 5.41 to 134.40 mg QE/g extract. Rosmarinic acid and chicoric acid contents varied from 0.118-1.234 and 0.014-0.597 μg/g DW, respectively. DPPH free radical scavenging activity varied from 76.12 to 513.5 μg/mL, while it ranged from 156.7 to 477.1 μg/mL with the ferrous ions (Fe) chelating assay. Rosmarinic acid and chicoric acid showed a significant and positive correlation ( = 0.81 and = 0.40) with total phenolics, respectively. The Ward dendrogram analysis revealed two different clusters based on the parameters measured, confirming high morpho-phytochemical variability among the individuals and populations. Principal component analysis (PCA) revealed eight PCs which contributed to 99.97% of the overall variance, and leaf length, essential oil content, and antioxidant activity in terms of DPPH and Fe chelating techniques were the most effective attributes for characterizing and selecting the studied population. Based on the traits related to vegetative yield and antioxidant properties, eight individuals from two populations were superior for breeding and/or farming programs.
刺芹（Trautv.）是伞形科一种分布广泛的植物，因其含有大量生物活性代谢物，如精油、酚类化合物和黄酮类化合物，而具有很高的营养价值和治疗特性。本研究旨在评估自然生长的刺芹种群的形态和生化变异性以及抗氧化特性。单因素方差分析表明，在所研究的种群中，大多数测量参数存在显著差异（P < 0.01）。鲜重范围为1.3至12.0克/株，干重范围为0.01至6.0克/株。精油产量的变异最大（变异系数=205.32%），其次是精油含量（变异系数=126.23%）和菊苣酸含量（变异系数=71.18%）。总酚含量在8.85至88.15毫克没食子酸当量/克提取物之间。总黄酮值在5.41至134.40毫克槲皮素当量/克提取物之间。迷迭香酸和菊苣酸含量分别在0.118 - 1.234和0.014 - 0.597微克/克干重之间。DPPH自由基清除活性在76.12至513.5微克/毫升之间，而亚铁离子（Fe）螯合试验的范围为156.7至477.1微克/毫升。迷迭香酸和菊苣酸分别与总酚呈显著正相关（r = 0.81和r = 0.40）。沃德聚类分析根据测量参数揭示了两个不同的聚类，证实了个体和种群之间存在高度的形态 - 植物化学变异性。主成分分析（PCA）揭示了八个主成分，它们占总方差的99.97%，就DPPH和Fe螯合技术而言，叶长、精油含量和抗氧化活性是表征和选择所研究种群的最有效属性。基于与营养产量和抗氧化特性相关的性状，来自两个种群的八株个体在育种和/或种植计划方面表现优异。
