疏肝和胃颗粒对非糜烂性反流病大鼠模型抑郁行为及内脏敏感性相关蛋白表达的影响
Effects of Shugan Hewei Granule on Depressive Behavior and Protein Expression Related to Visceral Sensitivity in a Rat Model of Nonerosive Reflux Disease.
作者信息
Wang Yi, Li Guanwu, Wang Xiaosu, Zhu Shengliang
机构信息
Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Department of Radiology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
出版信息
Evid Based Complement Alternat Med. 2019 Jan 2;2019:1505693. doi: 10.1155/2019/1505693. eCollection 2019.
OBJECTIVE
To explore the effect of Shugan Hewei Granule (SGHWG) and to provide the experimental basis for its clinical application.
METHODS
40 healthy male Wistar rats were divided into 5 groups, with 8 rats in each group, including control group, model group, normal saline (NS) group, SGHWG group, and Rabeprazole group. The control group was not treated. The model group was treated with fructose intake and mental stress to be the model of NERD. The other groups were treated as the model group and then gavaged with the corresponding drugs. The pH value of lower third of esophagus, immobile time in tail suspension test, CRF protein expression in both hypothalamus and anterior cingulate cortex (ACC), and SP protein in esophageal mucosa in lower third of esophagus detected by immunofluorescence and NMDAR1 protein expression in spinal cord detected by immunohistochemistry of each group were compared.
RESULTS
The pH values of both the SGHWG group and the Rabeprazole group were higher than that of the model group (<0.01), but the Rabeprazole group increased more obviously. The immobile time of the SGHWG group was shorter than that of the model group (<0.01) and the Rabeprazole group (<0.05). The expression of the CRF in the hypothalamus and ACC, NMDAR1 in the spinal cord, and SP in the esophageal mucosa in lower third of esophagus of the SGHWG group decreased significantly, compared with the model group (<0.01), and was obviously lower than that in the Rabeprazole group (<0.05).
CONCLUSIONS
This study provided an evidence that SGHW formula was inferior to Rabeprazole in acid inhibition, but it might reduce the expression of CRF protein of hypothalamus and ACC, lower the levels of NMDAR1 in spinal dorsal horn and SP in esophageal mucosa in lower third of esophagus, and regulate depressive behavior simultaneously, related to the improvement of visceral hypersensitivity in rat model of NERD.
目的
探讨疏肝和胃颗粒（SGHWG）的作用，为其临床应用提供实验依据。
方法
将40只健康雄性Wistar大鼠分为5组，每组8只，包括对照组、模型组、生理盐水（NS）组、SGHWG组和雷贝拉唑组。对照组不做处理。模型组通过摄入果糖和精神应激建立NERD模型。其他组按模型组处理后，给予相应药物灌胃。比较各组大鼠食管下三分之一处的pH值、悬尾试验中的不动时间、下丘脑和前扣带回皮质（ACC）中CRF蛋白表达、免疫荧光法检测食管下三分之一处食管黏膜中SP蛋白以及免疫组化法检测脊髓中NMDAR1蛋白表达。
结果
SGHWG组和雷贝拉唑组的pH值均高于模型组（<0.01），但雷贝拉唑组升高更明显。SGHWG组的不动时间短于模型组（<0.01）和雷贝拉唑组（<0.05）。与模型组相比，SGHWG组下丘脑和ACC中CRF的表达、脊髓中NMDAR1的表达以及食管下三分之一处食管黏膜中SP的表达均显著降低（<0.01），且明显低于雷贝拉唑组（<0.05）。
结论
本研究证明，SGHW方在抑酸作用上不如雷贝拉唑，但可能降低下丘脑和ACC中CRF蛋白的表达，降低脊髓背角NMDAR1水平以及食管下三分之一处食管黏膜中SP水平，同时调节抑郁行为，这与改善NERD大鼠模型的内脏高敏感性有关。
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