Diabetes and Metabolism Research Center, Division of Endocrinology, Diabetes & Metabolism, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
Division of General and Gastrointestinal Surgery, Department of Surgery, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
J Diabetes Res. 2019 Jan 3;2019:8124563. doi: 10.1155/2019/8124563. eCollection 2019.
Obesity is associated with a state of chronic low-grade inflammation both systemically and within specific tissues, including adipose tissue (AT). In murine models of obesity, there is a shift in the inflammatory profile of the AT immune cells, with an accumulation of proinflammatory M1 macrophages that surround the expanding adipocyte. However, much less is known about the immune cell composition and how to best define AT macrophages in humans. . The goals of the current study were to determine the contribution of macrophages to the stromal vascular fraction (SVF) in lean versus obese human visceral AT (VAT); examine the expression of common M1, M2, and pan macrophage markers; and determine the association of specific macrophage types with known biomarkers of obesity-related cardiometabolic disease. . VAT biopsies were obtained from obese ( = 50) and lean ( = 8) patients during elective surgery. Adipocytes and SVF were isolated, and the SVF was subjected to flow cytometry analyses. . Our results indicate that VAT macrophages are increased in obesity and associate with biomarkers of CVD but that many macrophages do not fall into currently defined M1/M2 classification system based on CD206 receptor expression levels. . VAT macrophages are increased in obese subjects, but the current markers used to define macrophage populations are inadequate to distinguish differences in human obesity. Further studies are needed to delineate the function of AT macrophages in the maintenance and progression of human AT inflammation in obesity.
肥胖与全身性和特定组织（包括脂肪组织）的慢性低度炎症状态有关。在肥胖的啮齿动物模型中，脂肪组织免疫细胞的炎症特征发生了转变，促炎 M1 巨噬细胞在不断扩张的脂肪细胞周围积聚。然而，对于人类脂肪组织中免疫细胞的组成以及如何最好地定义脂肪组织巨噬细胞，人们知之甚少。本研究的目的是确定巨噬细胞在肥胖者与瘦者内脏脂肪组织（VAT）基质血管部分（SVF）中的贡献；研究常见的 M1、M2 和巨噬细胞通用标志物的表达；以及确定特定巨噬细胞类型与肥胖相关的心血管代谢疾病的已知生物标志物之间的关联。通过选择性手术从肥胖者（=50）和瘦者（=8）患者中获得 VAT 活检。分离脂肪细胞和 SVF，并对 SVF 进行流式细胞术分析。结果表明，肥胖者的 VAT 巨噬细胞增加，并与 CVD 的生物标志物相关，但根据 CD206 受体表达水平，许多巨噬细胞不符合当前基于 M1/M2 分类系统的定义。肥胖者的 VAT 巨噬细胞增加，但目前用于定义巨噬细胞群体的标记物不足以区分人类肥胖中的差异。需要进一步研究来描绘脂肪组织巨噬细胞在肥胖中维持和促进人类脂肪组织炎症中的作用。
