Laboratório de Virologia Aplicada/Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, SC, 88040-900, Brazil.
Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.
Food Environ Virol. 2019 Jun;11(2):157-166. doi: 10.1007/s12560-019-09370-8. Epub 2019 Feb 4.
Millions of people use contaminated water sources for direct consumption. Chlorine is the most widely disinfection product but can produce toxic by-products. In this context, natural and synthetic compounds can be an alternative to water disinfection. Therefore, the aim of this study was to assess the inactivation of human adenovirus by N-chlorotaurine (NCT), bromamine-T (BAT) and Grape seed extract (GSE) in water. Distilled water artificially contaminated with recombinant human adenovirus type 5 (rAdV-GFP) was treated with different concentrations of each compound for up to 120 min, and viral infectivity was assessed by fluorescence microscopy. The decrease in activity of the compounds in the presence of organic matter was evaluated in water supplemented with peptone. As results, NCT and GSE inactivated approximately 2.5 log of adenovirus after 120 min. With BAT, more than 4.0 log decrease was observed within 10 min. The oxidative activity of 1% BAT decreased by 50% in 0.5% peptone within a few minutes, while the reduction was only 30% for 1% NCT in 5% peptone after 60 min. Organic matter had no effect on the activity of GSE. Moreover, the minimal concentration of BAT and GSE to kill viruses was lower than that known to kill human cells. It was concluded that the three compounds have potential to be used for water disinfection for drinking or reuse purposes.
数百万人直接使用受污染的水源。氯是最广泛使用的消毒剂，但会产生有毒副产物。在这种情况下，天然和合成化合物可以替代水消毒。因此，本研究旨在评估 N-氯代牛磺酸 (NCT)、溴化胺-T (BAT) 和葡萄籽提取物 (GSE) 在水中对人腺病毒的灭活作用。用不同浓度的每种化合物处理人工污染重组人腺病毒 5 型 (rAdV-GFP) 的蒸馏水长达 120 分钟，并通过荧光显微镜评估病毒感染力。在添加蛋白胨的水中评估了有机物存在下化合物活性的降低。结果表明，NCT 和 GSE 在 120 分钟后使腺病毒失活约 2.5 个对数。BAT 在 10 分钟内观察到超过 4.0 个对数的下降。在 0.5%蛋白胨中，1%BAT 的氧化活性在几分钟内降低了 50%，而在 60 分钟后，在 5%蛋白胨中，1%NCT 的减少仅为 30%。有机物对 GSE 的活性没有影响。此外，BAT 和 GSE 杀死病毒的最小浓度低于已知杀死人类细胞的浓度。因此，可以得出结论，这三种化合物具有用于饮用水或再利用目的的水消毒的潜力。
