Li Zhiyong, Jiang Danfeng, Yang Sheng
Department of Comprehensive Therapy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.
Department of Day Care, Beijing Cancer Hospital, Beijing, People's Republic of China.
Cancer Manag Res. 2020 Oct 30;12:10975-10984. doi: 10.2147/CMAR.S258182. eCollection 2020.
To investigate the effects of miR-490-3p on the proliferation, migration, invasion and apoptosis of lung adenocarcinoma (LUAD) cells through the Wnt/β-catenin signaling pathway.
Differentially expressed miRNAs in LUAD tissues were analyzed by bioinformatics and the target miRNA went through GSEA enrichment analysis. qRT-PCR was used to detect the expression of miR-490-3p in human LUAD cells and normal bronchial cells. The constructed vectors were transfected into the LUAD cell lines using Lipofectamine 2000. Cell viability was detected by MTT, cell migration and invasion were detected by transwell assay, and cell apoptosis was detected by flow cytometry. Western blot was performed to detect the expression levels of the proteins related to the Wnt/β-catenin pathway and cell apoptosis. Xenograft tumor mouse models were used for in vivo validation.
The results of qRT-PCR showed that miR-490-3p was relatively lowly expressed in LUAD cells, and the expression level was different in different LUAD cell lines. The results of MTT, transwell and flow cytometry exhibited that miR-490-3p could significantly inhibit the proliferation, migration, invasion and increase cell apoptosis rate of LUAD cells. Western blot results showed that miR-490-3p promoted the expression of Bax, Caspase-3 and E-cadherin as well as the phosphorylation of GSK-3β and inhibited the expression of Bcl-2, β-catenin and C-myc. Additionally, animal experiments were performed to prove that miR-490-3p suppressed LUAD malignant progression in vivo.
MiR-490-3p inhibited the proliferation, migration, invasion and promoted the apoptosis of LUAD cells by down-regulating the Wnt/β-catenin signaling pathway, suggesting that miR-490-3p may be an indicator for early diagnosis and prognosis of LUAD.
通过Wnt/β-连环蛋白信号通路研究miR-490-3p对肺腺癌（LUAD）细胞增殖、迁移、侵袭和凋亡的影响。
通过生物信息学分析LUAD组织中差异表达的miRNA，并对目标miRNA进行GSEA富集分析。采用qRT-PCR检测miR-490-3p在人LUAD细胞和正常支气管细胞中的表达。使用Lipofectamine 2000将构建的载体转染到LUAD细胞系中。通过MTT检测细胞活力，通过transwell实验检测细胞迁移和侵袭，通过流式细胞术检测细胞凋亡。进行蛋白质印迹法检测与Wnt/β-连环蛋白通路和细胞凋亡相关的蛋白质表达水平。采用异种移植瘤小鼠模型进行体内验证。
qRT-PCR结果显示，miR-490-3p在LUAD细胞中表达相对较低，且在不同的LUAD细胞系中表达水平不同。MTT、transwell和流式细胞术结果表明，miR-490-3p可显著抑制LUAD细胞的增殖、迁移、侵袭并提高细胞凋亡率。蛋白质印迹法结果显示，miR-490-3p促进Bax、Caspase-3和E-钙黏蛋白的表达以及GSK-3β的磷酸化，并抑制Bcl-2、β-连环蛋白和C-myc的表达。此外，进行动物实验证明miR-490-3p在体内抑制LUAD的恶性进展。
MiR-490-3p通过下调Wnt/β-连环蛋白信号通路抑制LUAD细胞的增殖、迁移、侵袭并促进其凋亡，提示miR-490-3p可能是LUAD早期诊断和预后的一个指标。
