Panezai Malala, Kamran Taj Muhammad, Nawaz Imran, Taj Imran, Panezai Marina, Panezai Nazia, Zafar Umbreen, Ghulam Muhammad Durdana, Ahmed Essote Saeed, Muhammad Ghulam
Pak J Biol Sci. 2018 Jan;21(9):469-474. doi: 10.3923/pjbs.2018.469.474.
Salmonella paratyphi cause enteric fever which is an important public health problem worldwide. In Pakistan incidence is increasing and affect all age groups. Therefore, the present research was designed to study the different microbiological aspects of Salmonella paratyphi.
The study was conducted to identify the Salmonella paratyphi from blood samples in Quetta. Total 480 blood samples were collected from different hospital of Quetta. Specific colony characters, microscopic examination, biochemical tests and PCR were used for identification of Salmonella paratyphi.
Total 55% samples were positive and 45% were negative for Salmonella paratyphi. Results showed that males (34%) were more affected with Salmonella paratyphi as compare to female (20%). Age wise distribution revealed that Salmonella paratyphi was high in 20-30 years (38%) followed by 10-20 years (9.16%) and 1-10 years (7.5%) age group patients. Paratyphoid fever cases were significantly high (25.41%) in Pashtoon population as compare to other population of Balochistan. The 40% paratyphoid fever was observed in the patients with low socioeconomic status, 9.16% in middle socioeconomic status and 5.83% in the patients belonged to high socioeconomic status. The Salmonella paratyphi were sensitive to Chloramphenicol (23 mm), Amikacin (24 mm), Gentamicin (12 mm), Quinolones (23) and Polypeptide (13 mm) classes. The PCR based identification of Salmonella paratyphi showed clear bands of 329 bp of flic-a gene.
To control paratyphoid fever strong initiatives must be taken to improve water sanitation, hygiene level, supply of save drinking water and vaccination is recommended in order to eradicate the disease.
甲型副伤寒沙门氏菌可引发伤寒热，这是一个全球性的重要公共卫生问题。在巴基斯坦，其发病率呈上升趋势，且影响所有年龄组。因此，本研究旨在探讨甲型副伤寒沙门氏菌的不同微生物学特征。
本研究旨在从奎达的血液样本中鉴定甲型副伤寒沙门氏菌。共从奎达的不同医院采集了480份血液样本。通过特定菌落特征、显微镜检查、生化试验及聚合酶链反应（PCR）来鉴定甲型副伤寒沙门氏菌。
甲型副伤寒沙门氏菌检测中，55%的样本呈阳性，45%呈阴性。结果显示，男性（34%）感染甲型副伤寒沙门氏菌的比例高于女性（20%）。按年龄分布显示，20至30岁年龄组患者感染甲型副伤寒沙门氏菌的比例最高（38%），其次是10至20岁（9.16%）和1至10岁（7.5%）年龄组。与俾路支省的其他人群相比，普什图族人群中的副伤寒热病例显著较高（25.41%）。在社会经济地位较低的患者中，40%出现副伤寒热，社会经济地位中等者为9.16%，社会经济地位较高者为5.83%。甲型副伤寒沙门氏菌对氯霉素（抑菌圈直径23毫米）、阿米卡星（24毫米）、庆大霉素（12毫米）、喹诺酮类（23毫米）和多肽类（13毫米）敏感。基于PCR的甲型副伤寒沙门氏菌鉴定显示出flic - a基因清晰的329碱基对条带。
为控制副伤寒热，必须采取有力举措改善水卫生、提高卫生水平、提供安全饮用水，并建议进行疫苗接种以根除该疾病。
