Abdallah Muhammad Salihu, Mustafa Muskhazli, Nallappan Meenakshii A/P, Choi Sangho, Paik Jin-Hyub, Rusea Go
Department of Biology, Universiti Putra Malaysia, Serdang, Malaysia.
Department of Microbiology, Yobe State University, Damaturu, Nigeria.
Front Chem. 2021 Jul 27;9:670530. doi: 10.3389/fchem.2021.670530. eCollection 2021.
Gallic acid and catechin are the most abundant phenolic and flavonoid contents found in all plant extracts. The contents and the bioassay-guided fractionating substances of the (A. Rich) Hochst (Anacardiaceae) fraction played vital roles. The goals of the study were to determine the contents of some useful medicinal plants and the bioassay-guided fractionation substances of fraction compounds capable of acting against isolate using LC-MS/LC-HRMS (Dionex ultimate 3000 RS UPLC with Thermo Scientific Q Exactive Orbitrap Hybrid Tandem Mass Spectrometer). The Folin-Ciocalteu reagent procedure and flavonoid content determination were conducted spectrophotometrically. Bioassay-guided fractionation, chronological partitioning, and screening of the antibacterial action against were performed. The ethyl acetate fraction extracts of stem (bark) extract were analyzed using LC-MS/LC-HRMS. The gallic acid content increased tremendously in (L.) P.J.H. Hurter and Mabb (Fabaceae) pod extracts with curve fitting ( = 0.9958). Catechin content increase was significantly increased in stem (bark) extracts followed by that of pod extracts with curve fitting ( = 0.9993); they were all significantly different in the J.F. Gmel. and the (Poir.) Goyder leaves extracts at value <0.0001. Subsequently, 10 mg/ml of stem (bark) ethyl acetate fraction extract was the MIC, where no MBC was recorded and susceptible to the positive control with the highest inhibition zone, followed by the ethyl acetate fraction extract at 10 mg/ml (9.7 ± 0.0) at Turkey's < 0.0001. Vidarabine is one of the novel compounds, specifically having antimicrobial actions, found in the stem (bark). Reasonable amounts of phenolic and flavonoid contents determined the actions of the individual plant extract.
没食子酸和儿茶素是所有植物提取物中含量最为丰富的酚类和黄酮类物质。（漆树科）提取物的含量及生物活性导向分离物质发挥了关键作用。本研究的目的是使用液相色谱-质谱联用仪/液相色谱-高分辨质谱仪（配备赛默飞世尔科技Q Exactive Orbitrap混合串联质谱仪的戴安UltiMate 3000 RS超高效液相色谱仪）测定一些有用药用植物的含量以及能够对抗分离株的提取物化合物的生物活性导向分离物质。采用福林-酚试剂法和分光光度法测定黄酮含量。进行了生物活性导向分离、时间顺序分配以及对其抗菌作用的筛选。使用液相色谱-质谱联用仪/液相色谱-高分辨质谱仪分析了茎（皮）提取物的乙酸乙酯部位提取物。通过曲线拟合，（豆科）豆荚提取物中的没食子酸含量大幅增加（= 0.9958）。茎（皮）提取物中的儿茶素含量显著增加，其次是豆荚提取物，通过曲线拟合（= 0.9993）；在值<0.0001时，它们在叶提取物和叶提取物中均存在显著差异。随后，10 mg/ml的茎（皮）乙酸乙酯部位提取物为最低抑菌浓度，未记录到最低杀菌浓度，且对阳性对照敏感，抑菌圈最大，其次是10 mg/ml（9.7±0.0）的乙酸乙酯部位提取物，在土耳其<0.0001。阿糖腺苷是在茎（皮）中发现的具有抗菌作用的新型化合物之一。合理含量的酚类和黄酮类物质决定了各个植物提取物的作用。
