1 Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
2 Faculty of Medicine, University of Tübingen, Tübingen, Germany.
Tissue Eng Part C Methods. 2019 Mar;25(3):127-136. doi: 10.1089/ten.TEC.2018.0356.
This study describes methods for fabricating, culturing, and characterizing modular microbeads containing progenitor cells that can be used to create osteochondral tissue constructs. Such biphasic engineered tissues were cultured in a low flow rate perfusion bioreactor chamber to maintain tissue-specific differentiation while allowing development of the osteochondral interface.
本研究描述了制造、培养和表征含有祖细胞的模块化微珠的方法，这些微珠可用于构建骨软骨组织构建体。这种双相工程组织在低流速灌注生物反应器室中进行培养，以维持组织特异性分化，同时允许骨软骨界面的发育。
