AMADH抑制剂的优化及其对NaCl-CaCl处理下大豆芽中GABA积累的影响。
AMADH inhibitor optimization and its effects on GABA accumulation in soybean sprouts under NaCl-CaCl treatment.
作者信息
Yang Runqiang, Wang Mian, Feng Xiaoyun, Gu Zhenxin, Wang Pei
机构信息
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.
出版信息
3 Biotech. 2019 May;9(5):184. doi: 10.1007/s13205-019-1715-7. Epub 2019 Apr 22.
ABSTRACT
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide (EDC) was the suitable inhibitor for aminoaldehyde dehydrogenase (AMADH) compared with -ethylmaleimide (NEE) and iodoacetamide (IAM). EDC exhibited the most obvious inhibition effect on AMADH activity, while its inhibition on glutamate decarboxylase (GAD) was insignificant. Compared with the control, AMADH activity reduced by 70.4% with 0.5 mM EDC, and γ-aminobutyric acid (GABA) content declined by 44.3% in soybean sprouts at 4 days of germination. AMADH activity reduced by 80.62, 67.61 and 72.02% in the 4-day sprouts with 1 mM EDC under NaCl, CaCl and NaCl + CaCl treatment, respectively, and GABA content decreased by 43.56, 38.84 and 35.53%, respectively. EDC is a proper inhibitor for AMADH and it could be used to quantify the contribution of polyamine degradation pathway on GABA formation. In soybean sprouts, the presence of CaCl under NaCl stress decreased the contribution of polyamine degradation pathway on GABA accumulation.
摘要
与N-乙基马来酰亚胺（NEE）和碘乙酰胺（IAM）相比，1-（3-二甲基氨基丙基）-3-乙基碳二亚胺（EDC）是氨基醛脱氢酶（AMADH）的合适抑制剂。EDC对AMADH活性表现出最明显的抑制作用，而对谷氨酸脱羧酶（GAD）的抑制作用不显著。与对照相比，0.5 mM EDC处理使发芽4天的豆芽中AMADH活性降低70.4%，γ-氨基丁酸（GABA）含量下降44.3%。在NaCl、CaCl和NaCl + CaCl处理下，1 mM EDC处理使4天龄豆芽中的AMADH活性分别降低80.62%、67.61%和72.02%，GABA含量分别下降43.56%、38.84%和35.53%。EDC是AMADH的合适抑制剂，可用于量化多胺降解途径对GABA形成的贡献。在豆芽中，NaCl胁迫下CaCl的存在降低了多胺降解途径对GABA积累的贡献。
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