Maurya Sudarshan, Naik S K, Choudhary J S, Kumar S
ICAR-Research Complex for Eastern Region, Research Centre, Ranchi, Jharkhand India.
Indian J Microbiol. 2019 Mar;59(1):27-38. doi: 10.1007/s12088-018-0756-7. Epub 2018 Aug 9.
(NAIMCC-F-03167) and (NAIMCC-F-03168) were isolated from the acidic soil of the vicinity of Litchi orchard, Ranchi, Jharkhand and were characterized on the basis of morphological, molecular and biochemical features. Both strains are fast growing, light to dark green, highly sporulative and have ability to cover 90 mm Petri dish within 96 h of inoculation. Biochemcial estimation of both isolates indicated significant cellulase and phosphate solubilisation activity. Highest cellulase activity was observed in . (5.63 cm) followed by . (5.10 cm) and phosphate solubilisation index was observed maximum in . (1.93) followed by . (1.39). Moreover, these isolates were molecularly identified on the basis of ribosomal DNA based sequences database and phylogenetic analysis in NCBI GenBank as . (NCBI-KM 438015) and . (NCBI-KJ910335). Negetive effect on sporulation of Lead (Pb) and Cadmium (Cd) was observed while in heavy metal scavenging potential, . (88.9% Cd) showed highest scavenging potential followed by . (87.2% Cd) while in Pb scavenging potential, . (88% Pb) followed highest scavenging potential followed by . (81.30% Pb) after 21 days of inoculation from 30 µg/ml heavy metals concentrated broth medium. If both potential bioagents can apply in Cd and Pb affected soil/water will be helpful in scavenging of heavy metals as well as management of phosphorus deficiency and soilborne fungal diseases.
(NAIMCC-F-03167) 和 (NAIMCC-F-03168) 从贾坎德邦兰契荔枝园附近的酸性土壤中分离得到，并根据形态学、分子学和生化特征进行了鉴定。两株菌株生长迅速，颜色从浅绿到深绿，产孢量大，接种后96小时内能长满90毫米的培养皿。对这两株分离菌的生化分析表明它们具有显著的纤维素酶活性和溶磷活性。在 (5.63厘米) 中观察到最高的纤维素酶活性，其次是 (5.10厘米)；在 (1.93) 中观察到最大的溶磷指数，其次是 (1.39)。此外，根据核糖体DNA序列数据库以及NCBI基因库中的系统发育分析，这些分离菌在分子水平上被鉴定为 (NCBI-KM 438015) 和 (NCBI-KJ910335)。观察到铅 (Pb) 和镉 (Cd) 对产孢有负面影响，而在重金属清除潜力方面，接种21天后，从30微克/毫升重金属浓缩肉汤培养基中观察到， (88.9% Cd) 具有最高的清除潜力，其次是 (87.2% Cd)；在铅清除潜力方面， (88% Pb) 具有最高的清除潜力，其次是 (81.30% Pb)。如果将这两种潜在的生物制剂应用于受镉和铅污染的土壤/水体中，将有助于清除重金属以及解决磷缺乏问题和土壤传播的真菌病害。
