来自KMJ820的纤维二糖水解酶的特性分析
Characterization of Cellobiohydrolases from KMJ820.
作者信息
Kondaveeti Sanath, Patel Sanjay K S, Woo Janghun, Wee Ji Hyang, Kim Sang-Yong, Al-Raoush Riyadh I, Kim In-Won, Kalia Vipin Chandra, Lee Jung-Kul
机构信息
1Department of Chemical Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 05029 Republic of Korea.
2Department of Food Science and Biotechnology, Shin-Ansan University, Ansan, 15435 Republic of Korea.
出版信息
Indian J Microbiol. 2020 Jun;60(2):160-166. doi: 10.1007/s12088-019-00843-9. Epub 2019 Nov 26.
A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51 U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using supernatants and two different CBHs were found; CBH 1 and 2. The filtered CBHs showed greater activity ( = 51.4 and 20.8 U/mg) in contrast to CBHs from earlier studies. The MW (molecular weights) of CBH 1 and 2 were verified to be approximately 50 kDa and 150 kDa, respectively, by size exclusion chromatography. Even though CBHs have been evaluated from other sources, but CBH is prominent in comparison to other CBHs by its high enzyme activity.
一种新型的产生纤维二糖水解酶（CBH）的真菌已被分离出来，并根据形态学和rDNA基因序列归类为KMJ820。以纤维素粉作为碳源，测得总酶活性为11.51 U/ml；这是所研究的产生CBH的微生物中酶活性最高的之一。已对CBH进行纯化以使其均质化，采用上清液进行了一系列色谱分析，发现了两种不同的CBH；即CBH 1和CBH 2。与早期研究中的CBH相比，经过滤的CBH显示出更高的活性（分别为51.4和20.8 U/mg）。通过尺寸排阻色谱法验证，CBH 1和CBH 2的分子量（MW）分别约为50 kDa和150 kDa。尽管已经对来自其他来源的CBH进行了评估，但该CBH因其高酶活性而比其他CBH更为突出。
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