来自某物种的基因的克隆、测序及电子分析
Cloning, Sequencing and In Silico Analysis of Gene from spp.
作者信息
Jangra Mukesh R, Batra Ritu, Passricha Nishat, Sikka Virendra K
机构信息
1Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar, India.
Bioinformatics Infrastructure Facility, Department of Genetics and Plant Breeding, CCSU, Meerut, India.
出版信息
Indian J Microbiol. 2019 Mar;59(1):58-63. doi: 10.1007/s12088-018-0767-4. Epub 2018 Nov 12.
We report here isolation and analysis of PCR amplified C gene from spp. strain phbmbb15-B3. This strain was previously developed from mutations of landfill isolates and found to be an efficient Poly Hydroxy butyrate (PHB) producer. The fragment was cloned into pTZ57R/T cloning vector and then the gene has been sequenced and submitted to GenBank (Accession Number KT933807). The sequence results confirmed the clone to be homologue and the ORF was 910 base pairs long and coded for 303 amino acids, which shared 92-99% amino acid sequence identity with the available bacterial sequences in Gene Bank. We could also predict the primary and secondary structural features of the expected protein. Phylogenetic analysis also revealed its similarity with several pseudomonads. The results of the present study shall provide a stable foundation for further research on modeling studies of PHB synthase and developing PHB a commercial technology.
我们在此报告从spp.菌株phbmbb15 - B3中分离并分析PCR扩增的C基因。该菌株先前由垃圾填埋场分离株的突变产生，并且被发现是一种高效的聚羟基丁酸酯（PHB）生产者。该片段被克隆到pTZ57R/T克隆载体中，然后对该基因进行了测序并提交到GenBank（登录号KT933807）。序列结果证实该克隆为同源物，其开放阅读框长910个碱基对，编码303个氨基酸，与基因库中现有的细菌序列具有92 - 99%的氨基酸序列同一性。我们还可以预测预期蛋白质的一级和二级结构特征。系统发育分析也揭示了它与几种假单胞菌的相似性。本研究结果将为进一步开展PHB合酶的建模研究以及开发PHB商业技术提供坚实的基础。
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