Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Department of Biotechnology, Savitribai Phule Pune University, Pune 411007, India.
Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Academy of Scientific & Innovative Research (AcSIR), 2, Rafi Marg, Anusandhan Bhawan, New Delhi 110001, India.
Bioresour Technol. 2016 Jan;200:413-9. doi: 10.1016/j.biortech.2015.10.045. Epub 2015 Oct 19.
Production of polyhydroxyalkanoate (PHA) co-polymers by Bacillus spp. was studied by feeding defined volatile fatty acids (VFAs) obtained through controlled hydrolysis of various wastes. Eleven mixed hydrolytic cultures (MHCs) each containing 6 strains could generate VFA from slurries of (2% total solids): pea-shells (PS), potato peels (PP), apple pomace (AP) and onion peels (OP). PS hydrolysates (obtained with MHC2 and MHC5) inoculated with Bacillus cereus EGU43 and Bacillus thuringiensis EGU45 produced co-polymers of PHA at the rate of 15-60mg/L with a 3HV content of 1%w/w. An enhancement in PHA yield of 3.66-fold, i.e. 205-550mg/L with 3HV content up to 7.5%(w/w) was observed upon addition of OP hydrolysate and 1% glucose (w/v) to PS hydrolysates. This is the first demonstration, where PHA co-polymer composition, under non-axenic conditions, could be controlled by customizing VFA profile of the hydrolysate by the addition of different biowastes.
采用经控制水解各种废物得到的挥发性脂肪酸（VFA）对 spp. 产生的聚羟基烷酸酯（PHA）共聚物进行了研究。11 种混合水解培养物（MHC）各包含 6 株，可以从（总固体含量为 2%）的悬浮液中产生 VFA：豌豆壳（PS）、土豆皮（PP）、苹果渣（AP）和洋葱皮（OP）。用 MHC2 和 MHC5 水解 PS 后，用蜡状芽孢杆菌 EGU43 和苏云金芽孢杆菌 EGU45 接种 PS 水解物，PHA 共聚物的生成率为 15-60mg/L，3HV 含量为 1%w/w。当向 PS 水解物中添加 OP 水解物和 1%葡萄糖（w/v）时，PHA 产量提高了 3.66 倍，即 205-550mg/L，3HV 含量高达 7.5%（w/w）。这是首次在非无菌条件下，通过添加不同生物废物来控制水解物的 VFA 组成，从而控制 PHA 共聚物组成的实例。
