Otari Sachin V, Patel Sanjay K S, Kim Sang-Yong, Haw Jung Rim, Kalia Vipin C, Kim In-Won, Lee Jung-Kul
1Division of Chemical Engineering, Konkuk University, Seoul, 05029 Republic of Korea.
2Department of Food Science and Biotechnology, Shin-Ansan University, Ansan, 15435 Republic of Korea.
Indian J Microbiol. 2019 Mar;59(1):105-108. doi: 10.1007/s12088-018-0768-3. Epub 2018 Nov 30.
In this study, novel, hollow superparamagnetic copper ferrite (CuFeO) nanoparticles (NPs) were synthesized by a low-temperature hydrothermal method. The hollow magnetic spheres were characterized by field emission scanning electron microscopy and high resolution transmission electron microscopy to confirm their morphology and size. The hollow NPs were demonstrated as the support for biological materials by the immobilization of lipase on the inner and outer surfaces of the hollow spheres. The immobilization of the enzyme was confirmed by Fourier Transform Infra-red spectroscopy and confocal laser scanning microscopy. The immobilized enzyme was shown to have an immobilization efficiency of 84.5%, with approximately 176 mg g of enzyme loading, for the hollow-NPs support. The immobilized enzyme exhibited high storage and temperature stability. The reusability of the immobilized lipase was more than 80% after 10 cycles of repeated use.
在本研究中，通过低温水热法合成了新型空心超顺磁性铜铁氧体（CuFeO）纳米颗粒（NPs）。用场发射扫描电子显微镜和高分辨率透射电子显微镜对空心磁球进行表征，以确认其形态和尺寸。通过将脂肪酶固定在空心球的内表面和外表面，证明了空心纳米颗粒可作为生物材料的载体。通过傅里叶变换红外光谱和共聚焦激光扫描显微镜确认了酶的固定化。对于空心纳米颗粒载体，固定化酶的固定效率为84.5%，酶负载量约为176 mg/g。固定化酶表现出高储存稳定性和热稳定性。固定化脂肪酶在重复使用10个循环后，其重复使用性超过80%。
