用于固定化酶的铜铁氧体磁性纳米颗粒。
Copper Ferrite Magnetic Nanoparticles for the Immobilization of Enzyme.
作者信息
Otari Sachin V, Patel Sanjay K S, Kim Sang-Yong, Haw Jung Rim, Kalia Vipin C, Kim In-Won, Lee Jung-Kul
机构信息
1Division of Chemical Engineering, Konkuk University, Seoul, 05029 Republic of Korea.
2Department of Food Science and Biotechnology, Shin-Ansan University, Ansan, 15435 Republic of Korea.
出版信息
Indian J Microbiol. 2019 Mar;59(1):105-108. doi: 10.1007/s12088-018-0768-3. Epub 2018 Nov 30.
Abstract
In this study, novel, hollow superparamagnetic copper ferrite (CuFeO) nanoparticles (NPs) were synthesized by a low-temperature hydrothermal method. The hollow magnetic spheres were characterized by field emission scanning electron microscopy and high resolution transmission electron microscopy to confirm their morphology and size. The hollow NPs were demonstrated as the support for biological materials by the immobilization of lipase on the inner and outer surfaces of the hollow spheres. The immobilization of the enzyme was confirmed by Fourier Transform Infra-red spectroscopy and confocal laser scanning microscopy. The immobilized enzyme was shown to have an immobilization efficiency of 84.5%, with approximately 176 mg g of enzyme loading, for the hollow-NPs support. The immobilized enzyme exhibited high storage and temperature stability. The reusability of the immobilized lipase was more than 80% after 10 cycles of repeated use.
摘要
在本研究中，通过低温水热法合成了新型空心超顺磁性铜铁氧体（CuFeO）纳米颗粒（NPs）。用场发射扫描电子显微镜和高分辨率透射电子显微镜对空心磁球进行表征，以确认其形态和尺寸。通过将脂肪酶固定在空心球的内表面和外表面，证明了空心纳米颗粒可作为生物材料的载体。通过傅里叶变换红外光谱和共聚焦激光扫描显微镜确认了酶的固定化。对于空心纳米颗粒载体，固定化酶的固定效率为84.5%，酶负载量约为176 mg/g。固定化酶表现出高储存稳定性和热稳定性。固定化脂肪酶在重复使用10个循环后，其重复使用性超过80%。
相似文献
1
Copper Ferrite Magnetic Nanoparticles for the Immobilization of Enzyme.用于固定化酶的铜铁氧体磁性纳米颗粒。
Indian J Microbiol. 2019 Mar;59(1):105-108. doi: 10.1007/s12088-018-0768-3. Epub 2018 Nov 30.
2
Preparation Fe3O4@chitosan magnetic particles for covalent immobilization of lipase from Thermomyces lanuginosus.制备用于共价固定嗜热栖热菌脂肪酶的Fe3O4@壳聚糖磁性颗粒。
Int J Biol Macromol. 2015 Apr;75:44-50. doi: 10.1016/j.ijbiomac.2015.01.020. Epub 2015 Jan 17.
3
Immobilization of Candida antarctica Lipase B on Magnetic Poly(Urea-Urethane) Nanoparticles.南极假丝酵母脂肪酶B固定于磁性聚（脲 - 聚氨酯）纳米颗粒上。
Appl Biochem Biotechnol. 2016 Oct;180(3):558-575. doi: 10.1007/s12010-016-2116-6. Epub 2016 May 16.
4
Covalent immobilization of porcine pancreatic lipase on carboxyl-activated magnetic nanoparticles: characterization and application for enzymatic inhibition assays.猪胰脂肪酶的羧基化磁性纳米粒子共价固定化：表征及其在酶抑制测定中的应用。
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:278-85. doi: 10.1016/j.msec.2014.02.011. Epub 2014 Feb 15.
5
Immobilization of lipase onto micron-size magnetic beads.将脂肪酶固定于微米级磁珠上。
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 5;822(1-2):91-7. doi: 10.1016/j.jchromb.2005.06.001.
6
A facile and effective immobilization of glucose oxidase on tannic acid modified CoFe2O4 magnetic nanoparticles.简便有效地将葡萄糖氧化酶固定在单宁酸修饰的 CoFe2O4 磁性纳米粒子上。
Colloids Surf B Biointerfaces. 2015 Dec 1;136:963-70. doi: 10.1016/j.colsurfb.2015.10.053. Epub 2015 Nov 4.
7
Effect of size and silica coating on structural, magnetic as well as cytotoxicity properties of copper ferrite nanoparticles.粒径和二氧化硅包覆对铜铁氧体纳米颗粒结构、磁性和细胞毒性的影响。
Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:552-566. doi: 10.1016/j.msec.2018.12.051. Epub 2018 Dec 19.
8
Rational Design of Nanoparticle Platforms for "Cutting-the-Fat": Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles.用于“减脂”的纳米颗粒平台的合理设计：脂肪酶、甘油激酶和甘油-3-磷酸氧化酶在金属纳米颗粒上的共价固定
Methods Enzymol. 2016;571:197-223. doi: 10.1016/bs.mie.2016.01.022.
9
Magnetic FeO@MCM-41 core-shell nanoparticles functionalized with thiol silane for efficient l-asparaginase immobilization.巯基硅烷功能化的磁性 FeO@MCM-41 核壳纳米粒子用于高效固定化 l-天冬酰胺酶。
Artif Cells Nanomed Biotechnol. 2018;46(sup2):1035-1045. doi: 10.1080/21691401.2018.1478422. Epub 2018 Jun 6.
10
Immobilization of Aspergillus niger lipase on chitosan-coated magnetic nanoparticles using two covalent-binding methods.采用两种共价结合方法将黑曲霉脂肪酶固定在壳聚糖包被的磁性纳米颗粒上。
Bioprocess Biosyst Eng. 2015 Aug;38(8):1437-45. doi: 10.1007/s00449-015-1385-8. Epub 2015 Mar 11.
引用本文的文献
1
Biogenic synthesis of AuNPs using L. and their antibacterial, antioxidant and catalytic applications.利用[细菌名称未完整给出]生物合成金纳米粒子及其抗菌、抗氧化和催化应用。
Indian J Microbiol. 2023 Dec;63(4):562-574. doi: 10.1007/s12088-023-01114-4. Epub 2023 Nov 3.
2
Emerging Field of Nanotechnology in Environment.纳米技术在环境领域的新兴领域。
Indian J Microbiol. 2023 Sep;63(3):244-252. doi: 10.1007/s12088-023-01092-7. Epub 2023 Sep 2.
3
Novel Biocompatible Green Silver Nanoparticles Efficiently Eliminates Multidrug Resistant Nosocomial Pathogens and Mycobacterium Species.新型生物相容性绿色银纳米颗粒可有效消除多重耐药医院病原体和分枝杆菌属物种。
Indian J Microbiol. 2023 Mar;63(1):73-83. doi: 10.1007/s12088-023-01061-0. Epub 2023 Feb 6.
4
Laccase Immobilization on Copper-Magnetic Nanoparticles for Efficient Bisphenol Degradation.漆酶固定在铜-磁性纳米粒子上用于高效双酚降解。
J Microbiol Biotechnol. 2023 Jan 28;33(1):127-134. doi: 10.4014/jmb.2210.10032. Epub 2022 Nov 11.
5
Expanding the Scope of Nanobiocatalysis and Nanosensing: Applications of Nanomaterial Constructs.拓展纳米生物催化与纳米传感的范围：纳米材料构建体的应用
ACS Omega. 2022 Sep 8;7(37):32863-32876. doi: 10.1021/acsomega.2c03155. eCollection 2022 Sep 20.
6
Therapeutic Applications of Self-assembled Indole-3-butanoyl-polyethylenimine Nanostructures.自组装吲哚-3-丁酰基-聚乙烯亚胺纳米结构的治疗应用
Indian J Microbiol. 2022 Sep;62(3):411-418. doi: 10.1007/s12088-022-01015-y. Epub 2022 Apr 18.
7
Evaluation of TiO Nanoparticles Physicochemical Parameters Associated with their Antimicrobial Applications.与TiO纳米颗粒抗菌应用相关的物理化学参数评估。
Indian J Microbiol. 2022 Sep;62(3):338-350. doi: 10.1007/s12088-022-01018-9. Epub 2022 Apr 18.
8
Nano-Therapeutics to Treat Acne Vulgaris.用于治疗寻常痤疮的纳米疗法。
Indian J Microbiol. 2022 Jun;62(2):167-174. doi: 10.1007/s12088-022-01001-4. Epub 2022 Jan 29.
9
Immobilization of Laccase Through Inorganic-Protein Hybrids Using Various Metal Ions.利用多种金属离子通过无机-蛋白质杂化物固定漆酶
Indian J Microbiol. 2022 Jun;62(2):312-316. doi: 10.1007/s12088-022-01000-5. Epub 2022 Jan 27.
10
Antimicrobial and Antioxidant Potential of Extract Coated AuNPs.提取物包覆金纳米粒子的抗菌和抗氧化潜力
Indian J Microbiol. 2021 Dec;61(4):506-518. doi: 10.1007/s12088-021-00976-w. Epub 2021 Sep 7.
本文引用的文献
1
Preparation of porous hollow FeO/P(GMA-DVB-St) microspheres and application for lipase immobilization.多孔空心 FeO/P(GMA-DVB-St)微球的制备及其在脂肪酶固定化中的应用。
Bioprocess Biosyst Eng. 2018 Jun;41(6):771-779. doi: 10.1007/s00449-018-1910-7. Epub 2018 Feb 14.
2
Synthesis of Protein-Inorganic Nanohybrids with Improved Catalytic Properties Using Co(PO).使用磷酸钴合成具有改进催化性能的蛋白质-无机纳米杂化物
Indian J Microbiol. 2018 Mar;58(1):100-104. doi: 10.1007/s12088-017-0700-2. Epub 2017 Dec 30.
3
Immobilization of Xylanase Using a Protein-Inorganic Hybrid System.使用蛋白质-无机杂化系统固定化木聚糖酶
J Microbiol Biotechnol. 2018 Apr 28;28(4):638-644. doi: 10.4014/jmb.1710.10037.
4
SnO hollow nanotubes: a novel and efficient support matrix for enzyme immobilization.SnO 空心纳米管：一种新型且高效的酶固定化支撑基质。
Sci Rep. 2017 Nov 10;7(1):15333. doi: 10.1038/s41598-017-15550-y.
5
Using concanavalinA as a spacer for immobilization of E. coli onto magnetic nanoparticles.使用伴刀豆球蛋白A作为间隔物将大肠杆菌固定在磁性纳米颗粒上。
Int J Biol Macromol. 2017 Nov;104(Pt A):63-69. doi: 10.1016/j.ijbiomac.2017.05.150. Epub 2017 May 27.
6
Eco-Friendly Composite of FeO-Reduced Graphene Oxide Particles for Efficient Enzyme Immobilization.用于高效酶固定化的 FeO 还原氧化石墨烯颗粒的环保复合材料。
ACS Appl Mater Interfaces. 2017 Jan 25;9(3):2213-2222. doi: 10.1021/acsami.6b05165. Epub 2017 Jan 10.
7
A highly efficient sorbitol dehydrogenase from Gluconobacter oxydans G624 and improvement of its stability through immobilization.一株氧化葡萄糖酸杆菌 G624 来源的高效山梨醇脱氢酶及其固定化提高其稳定性。
Sci Rep. 2016 Sep 16;6:33438. doi: 10.1038/srep33438.
8
Immobilization of Lipase by Adsorption Onto Magnetic Nanoparticles in Organic Solvents.在有机溶剂中通过吸附到磁性纳米颗粒上固定化脂肪酶。
J Nanosci Nanotechnol. 2016 Jan;16(1):601-7. doi: 10.1166/jnn.2016.10694.
9
Large-scale aerosol-assisted synthesis of biofriendly Fe₂O₃ yolk-shell particles: a promising support for enzyme immobilization.大规模气溶胶辅助合成生物友好型 Fe₂O₃ 蛋黄壳颗粒：酶固定化的一种有前途的载体。
Nanoscale. 2016 Mar 28;8(12):6728-38. doi: 10.1039/c6nr00346j.
10
Preparation Fe3O4@chitosan magnetic particles for covalent immobilization of lipase from Thermomyces lanuginosus.制备用于共价固定嗜热栖热菌脂肪酶的Fe3O4@壳聚糖磁性颗粒。
Int J Biol Macromol. 2015 Apr;75:44-50. doi: 10.1016/j.ijbiomac.2015.01.020. Epub 2015 Jan 17.
Zotero 插件