Singh Lalita, Antil Reena, Dahiya Pushpa
Department of Botany, Maharshi Dayanand University Rohtak, Haryana, India.
Indian J Microbiol. 2021 Dec;61(4):506-518. doi: 10.1007/s12088-021-00976-w. Epub 2021 Sep 7.
Green synthesis of nanoparticles is an important tool to reduce the harmful effects associated with traditional methods. In the present investigation, we have synthesised gold nanoparticles (AuNPs) using aqueous extract prepared from fresh aerial parts (leaf and stem) of as bioreducing agent. The visual indication of change in colour from pale yellow to brown to ruby-red indicated the successful formation of the AuNPs. Characterization of nanoparticles was carried out by UV-visible spectroscopy, X-ray crystallography (XRD), Transmission electron microscopy (TEM) and Energy dispersive X-ray analysis (EDX). UV-Vis spectra showed a specific peak at 546 nm which was the initial confirmation of the biosynthesized AuNPs. TEM images showed spherical and triangular shape of AuNPs with an average size of 25 nm. From FTIR spectrum, different functional groups were identified that could be responsible for the formation, stabilization, and capping of biosynthesized AuNPs. Aqueous plant extract and biosynthesised AuNPs were separately tested for their antimicrobial activity against six bacterial strains and four fungal strains. Biosynthesised AuNPs (2 mg/ml) showed significantly high zone of inhibition against the selected bacterial strains as compared to the aqueous plant extract. Maximum zone of inhibition (18.2 mm) was observed with AuNPs against whereas comparatively less value (12.5 mm) was recorded with the plant extract. Interestingly, the inhibitory activity observed against bacterial strains was even better than ampicillin. Antifungal activity recorded with AuNPs (5 mg/ml) was maximum (17.4 mm) against and it was higher than positive control (17.00 mm) and plant extract (13.2 mm).The present study clearly showed that AuNPs coated with extract were as good as positive control in inhibiting bacterial and fungal growth. In addition, these AuNPs also showed good antioxidant potential which was comparable to ascorbic acid.
The online version contains supplementary material available at 10.1007/s12088-021-00976-w.
纳米颗粒的绿色合成是一种减少传统方法相关有害影响的重要手段。在本研究中，我们使用从[植物名称]新鲜地上部分（叶和茎）制备的水提取物作为生物还原剂合成了金纳米颗粒（AuNPs）。颜色从浅黄色变为棕色再变为红宝石红色的视觉变化表明AuNPs成功形成。通过紫外可见光谱、X射线晶体学（XRD）、透射电子显微镜（TEM）和能量色散X射线分析（EDX）对纳米颗粒进行了表征。紫外可见光谱在546nm处显示出一个特定峰，这是生物合成AuNPs的初步确认。TEM图像显示AuNPs呈球形和三角形，平均尺寸为25nm。从傅里叶变换红外光谱（FTIR）中，鉴定出了不同的官能团，它们可能负责生物合成AuNPs的形成、稳定和包覆。分别测试了植物水提取物和生物合成的AuNPs对六种细菌菌株和四种真菌菌株的抗菌活性。与植物水提取物相比，生物合成的AuNPs（2mg/ml）对所选细菌菌株显示出显著更高的抑菌圈。AuNPs对[细菌名称]观察到的最大抑菌圈为（18.2mm），而植物提取物记录的值相对较小（12.5mm）。有趣的是，观察到的对细菌菌株的抑制活性甚至优于氨苄青霉素。AuNPs（5mg/ml）记录的抗真菌活性对[真菌名称]最大（17.4mm），且高于阳性对照（17.00mm）和植物提取物（13.2mm）。本研究清楚地表明，用[植物名称]提取物包覆的AuNPs在抑制细菌和真菌生长方面与阳性对照一样好。此外，这些AuNPs还显示出良好的抗氧化潜力，与抗坏血酸相当。
在线版本包含可在10.1007/s12088-021-00976-w获取的补充材料。
