通过B38.3提高诺索霉素A产量的基因组工程方法
Genome Engineering Approaches to Improve Nosokomycin A Production by B38.3.
作者信息
Kuzhyk Yuriy, Lopatniuk Maria, Luzhetskyy Andriy, Fedorenko Victor, Ostash Bohdan
机构信息
1Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Hrushevskoho St. 4, Rm. 102, Lviv, 79005 Ukraine.
2Actinobacteria Metabolic Engineering Group, Helmholtz-Institute for Pharmaceutical Research Saarland, Saarland University, Campus C2 3, 66123 Saarbrücken, Germany.
出版信息
Indian J Microbiol. 2019 Mar;59(1):109-111. doi: 10.1007/s12088-018-0761-x. Epub 2018 Sep 25.
Abstract
Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.
摘要
在此，我们描述了通过基因组工程方法提高ATCC14672生产磷酸糖脂抗生素诺索霉素A水平的努力。引入两个额外拷贝的亮氨酰tRNA（UUA）基因和一个拷贝的默诺霉素生物合成基因簇，平均使诺索霉素A的效价提高了三倍（高达1.5毫克/升）。我们的结果验证了基因组工程方法是提高默诺霉素产量的可行策略。
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