Pourali Parastoo, Yahyaei Behrooz
Department of Medical Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
3 Biotech. 2019 Feb;9(2):59. doi: 10.1007/s13205-019-1588-9. Epub 2019 Jan 30.
was used for production of bacterial cellulose (BC). The obtained BC was washed and floated in the nontoxic dose of the herb aqueous extract that was obtained from the methyl thiazol tetrazolium (MTT) assay. Twenty-four Wistar rats were divided into four separated groups and after inducing the wounds (15 mm in diameter), each group was treated with honey, BC, herb aqueous extract, and the combination of BC-herb aqueous extract. Each day the contraction percentages of the wound sites were measured. On days 3, 7, and 14, two rats from each group were euthanized, the skin samples from the wound regions were achieved and their paraffin blocks were prepared. Finally after trichrome staining, the microscopic examinations were done. MTT assay results indicated that the herb aqueous extract had dose-dependent toxic effects and the nontoxic dose of the extract was prepared and utilized for in vivo assay. Although the macroscopic analysis revealed that the BC and the herb aqueous extract had better activity in the wound contraction percentages than their combination, microscopic analysis indicated that the combination of BC-herb aqueous extract revealed all the characteristics that each material induced in the wound site alone. In conclusion, the speed of the wound healing should not be solely considered and its quality should be considered as well.
用于生产细菌纤维素（BC）。将获得的BC洗涤后，置于从甲基噻唑四氮唑蓝（MTT）测定中获得的无毒剂量的草药水提取物中漂浮。将24只Wistar大鼠分为四组，在诱导伤口（直径15毫米）后，每组分别用蜂蜜、BC、草药水提取物以及BC - 草药水提取物组合进行治疗。每天测量伤口部位的收缩百分比。在第3、7和14天，每组处死两只大鼠，获取伤口区域的皮肤样本并制备石蜡块。最后，经过三色染色后进行显微镜检查。MTT测定结果表明，草药水提取物具有剂量依赖性毒性作用，制备了提取物的无毒剂量并用于体内试验。尽管宏观分析显示BC和草药水提取物在伤口收缩百分比方面比它们的组合具有更好的活性，但显微镜分析表明，BC - 草药水提取物组合展现出了每种材料单独在伤口部位所诱导的所有特征。总之，伤口愈合的速度不应被单独考虑，其质量也应被考虑在内。
