Terpiłowska Sylwia, Siwicki Andrzej Krzysztof
Laboratory of Environmental Biology, Institute of Environmental Engineering, The John Paul II Catholic University of Lublin, Racławickie 14 Av, 20-950, Lublin, Poland.
Department of Microbiology and Clinical Immunology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, Str., 10-957, Olsztyn, Poland.
Biometals. 2017 Aug;30(4):565-574. doi: 10.1007/s10534-017-0027-9. Epub 2017 Jun 13.
The aim of this study was to examine the effect of treating of chromium(III) and iron(III) and their combinations on Herpes Simplex Virus type 1 (HSV-1) and Bovine Viral Diarrhoea virus (BVDV) replication. The antiviral efficacies of chromium(III) and iron(III) on HSV-1 and BVDV were evaluated using Real Time PCR method. Moreover, the cytotoxicity of these microelements was examined using the MTT reduction assay. The IC (50% inhibiotory concentration) for the chromium chloride was 1100 μM for Hep-2 cells and 1400 μM for BT cells. The IC for the iron chloride was 1200 μM for Hep-2 cells and more than1400 μM for BT cells. The concentration-dependent antiviral activity of chromium chloride and iron chloride against HSV-1 and BVDV viruses was observed. In cultures simultaneously treated with (1) 200 μM of CrCl and 1000 μM of FeCl, (2) 1000 μM of CrCl and 200 μM of FeCl, (3) 400 μM of CrCl and 800 μM of FeCl, (4) 800 μM of CrCl and 400 μM of FeCl a decrease in number of DNA or RNA copies was observed compared with control cells and cells incubated with chromium(III) and iron(III) used separately. The synergistic antiviral effects were observed for chromium(III) and iron(III) against HSV-1 and BVDV.
本研究的目的是检测铬（III）和铁（III）及其组合对1型单纯疱疹病毒（HSV-1）和牛病毒性腹泻病毒（BVDV）复制的影响。使用实时荧光定量PCR方法评估铬（III）和铁（III）对HSV-1和BVDV的抗病毒效果。此外，使用MTT还原试验检测这些微量元素的细胞毒性。氯化铬对Hep-2细胞的IC（50%抑制浓度）为1100μM，对BT细胞为1400μM。氯化铁对Hep-2细胞的IC为1200μM，对BT细胞大于1400μM。观察到氯化铬和氯化铁对HSV-1和BVDV病毒具有浓度依赖性抗病毒活性。在同时用（1）200μM CrCl和1000μM FeCl、（2）1000μM CrCl和200μM FeCl、（3）400μM CrCl和800μM FeCl、（4）800μM CrCl和400μM FeCl处理的培养物中，与对照细胞以及单独用铬（III）和铁（III）孵育的细胞相比，观察到DNA或RNA拷贝数减少。观察到铬（III）和铁（III）对HSV-1和BVDV具有协同抗病毒作用。
