Nedashkovskaya Olga I, Kim Song-Gun, Zhukova Natalia V, Mikhailov Valery V
G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.
Far Eastern Federal University, Sukhanova St. 8, 690950, Vladivostok, Russia.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2205-2210. doi: 10.1099/ijsem.0.001926. Epub 2017 Jul 12.
A strictly aerobic, Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 3Alg 18T, was isolated from the Pacific green alga Ulva fenestrata. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was affiliated to the family Flavobacteriaceae of the phylum Bacteroidetes, being most closely related to the type strains of recognized species of the genus Olleya, with 16S rRNA gene sequence similarity of 97.9-99.3 %. Strain 3Alg 18T grew in the presence of 0.5-5 % (w/v) NaCl and at 4-37 °C, and hydrolysed aesculin, casein, gelatin, starch and Tweens 20, 40 and 80. The prevalent fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C16:0 2-OH, iso-C17 : 0 3-OH, summed feature 3, iso-C16 : 0 3-OH, anteiso-C15 : 0 and C15 : 0. The polar lipid profile contained phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The major respiratory quinone was menaquinone MK-6. The genomic DNA G+C content was 34.6 mol%. On the basis of 16S rRNA gene sequence data, and chemotaxonomic and phenotypic characteristics, strain 3Alg 18T represents a novel species of the genus Olleya, for which the name Olleya algicola sp. nov. is proposed. The type strain is 3Alg 18T (=KCTC 22024T=KMM 6133T).
从太平洋绿藻孔石莼中分离出一株严格需氧、革兰氏染色阴性、杆状、靠滑动运动且产黄色色素的细菌，命名为菌株3Alg 18T。基于16S rRNA基因序列的系统发育分析表明，该新菌株隶属于拟杆菌门黄杆菌科，与Olleya属已确认种的模式菌株关系最为密切，16S rRNA基因序列相似性为97.9 - 99.3%。菌株3Alg 18T在0.5 - 5%（w/v）NaCl存在的条件下以及4 - 37°C温度下生长，能水解七叶苷、酪蛋白、明胶、淀粉以及吐温20、40和80。主要脂肪酸为异C15 : 0、异C15 : 1 G、异C15 : 0 3 - OH、异C16:0 2 - OH、异C17 : 0 3 - OH、特征性总和3、异C16 : 0 3 - OH、anteiso - C15 : 0和C15 : 0。极性脂质谱包含磷脂酰乙醇胺、三种未鉴定的氨基脂质和四种未鉴定的脂质。主要呼吸醌为甲基萘醌MK - 6。基因组DNA G + C含量为34.6 mol%。基于16S rRNA基因序列数据以及化学分类和表型特征，菌株3Alg 18T代表Olleya属的一个新物种，为此提出新名称Olleya algicola sp. nov.。模式菌株为3Alg 18T（=KCTC 22024T=KMM 6133T）。
