Isolation and Generation of Osteoclasts.
作者信息
Rucci Nadia, Zallone Alberta, Teti Anna
机构信息
Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy.
Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari, Bari, Italy.
出版信息
Methods Mol Biol. 2019;1914:3-19. doi: 10.1007/978-1-4939-8997-3_1.
PMID:30729457
Abstract
This chapter describes the isolation, culture, and staining of osteoclasts. The key advantages of this assay are that it allows direct measurement of osteoclast number, bone resorption, as well as yielding good quantities of osteoclasts at defined stages of formation for molecular analysis. An additional focus of this chapter will be the generation of osteoclasts from less conventional animal species and cell lines.
摘要
Zotero 插件