Brimer L
Department of Pharmacognosy and Botany, Royal Danish School of Pharmacy, Copenhagen, Denmark.
Ciba Found Symp. 1988;140:177-200. doi: 10.1002/9780470513712.ch11.
A survey of methods for the qualitative and quantitative determination of cyanide and cyanogenic compounds is presented. Particular attention is paid to determination in complex matrices. Chromatographic methods able to separate mixtures of closely related structures, such as glycosides of enantiomeric hydroxynitriles or lipids with slightly differing fatty acid spectra, are included, as are highly selective methods of detection, such as enzymic post-column cleavage combined with electrochemical detection as used in high-performance liquid chromatography. Details of thin-layer chromatography, including methods for detection, are given for both straight-phase and reverse-phase systems. The survey includes simple field methods as well as automated laboratory methods for the determination of 'free', 'bound' and 'total' cyanide, for example in processed food products from Manihot esculenta Cranz. Sources of enzymes are listed and attention is given to problems of sample storage and preparation. References are given to review articles which include data from methods such as ultraviolet, infrared and proton and carbon-13 nuclear magnetic resonance spectroscopies.
本文介绍了氰化物和氰基化合物定性和定量测定方法的综述。特别关注复杂基质中的测定。其中包括能够分离结构密切相关的混合物的色谱方法，例如对映体羟基腈的糖苷或脂肪酸谱略有不同的脂质，以及高选择性检测方法，如高效液相色谱中使用的酶柱后裂解结合电化学检测。给出了正相和反相系统的薄层色谱细节，包括检测方法。该综述包括简单的现场方法以及用于测定“游离”、“结合”和“总”氰化物的自动化实验室方法，例如来自木薯（Manihot esculenta Cranz）的加工食品。列出了酶的来源，并关注样品储存和制备问题。还给出了参考文献，这些综述文章包含来自紫外、红外、质子和碳-13核磁共振光谱等方法的数据。
