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库尔特计数器-化学法对原料乳体细胞计数的协作研究

Collaborative Study of the Coulter Counter-Chemical Method for Counting Somatic Cells in Raw Milk .

作者信息

Ginn R E, Thompson D R, Packard V S

机构信息

Dairy Quality Control Institute, Inc., St. Paul, Minnesota 55113, and Department of Agricultural Engineering and Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota 55108.

出版信息

J Food Prot. 1977 Jul;40(7):456-458. doi: 10.4315/0362-028X-40.7.456.

DOI:10.4315/0362-028X-40.7.456
PMID:30731619
Abstract

Variation between laboratories for Electronic Somatic Cell Counting by the chemical method (ESCC) was evaluated by a collaborative study. Eight laboratories counted somatic cells in 12 milk samples (six replicated samples) by the ESCC method. The somatic cell count for the same milk samples was also evaluated by the Direct Microscopic Somatic Cell Counting procedure (DMSCC) as a comparison for the level of error. The standard deviation of the variation of logarithms of ESCC counts between laboratories was 0.04368. The standard deviation for the variation of logarithms of DMSCC counts between technicians was 0.08617. The corresponding value for the DMSCC analysis of the last set of federal split milk samples was 0.141. An earlier study of electronic counting by the centrifuge method showed a standard deviation of 0.0711.

摘要

通过一项协作研究评估了各实验室之间采用化学方法进行电子体细胞计数(ESCC)的差异。八个实验室采用ESCC方法对12份牛奶样本(六个重复样本)中的体细胞进行计数。同时,采用直接显微镜体细胞计数法(DMSCC)对相同牛奶样本的体细胞计数进行评估,以比较误差水平。各实验室之间ESCC计数对数变化的标准差为0.04368。技术人员之间DMSCC计数对数变化的标准差为0.08617。对最后一组联邦分割牛奶样本进行DMSCC分析的相应值为0.141。一项早期关于离心法电子计数的研究显示标准差为0.0711。

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