Kelley W N
J Assoc Off Anal Chem. 1978 Nov;61(6):1328-34.
A collaborative study was conducted to compare the improved automated optical somatic cell counting procedure (OSCC II) with the direct microscopic somatic cell counting method (DMSCC) in raw milk. Samples were prefixed with formaldehyde and introduced into an Auto-Analyzer system. Dilution, clarification, and cell counting were performed automatically. Five collaborators participated in the study; they analyzed 48 samples in duplicate, using 2 different sampling rates. The results were compared with DMSCC counts reported by 3 different analysts. Statistical results show that the standard deviation for the DMSCC method is 0.0825 and for the OSCC II, at a sampling rate of 80/hr, 0.0434. When results are compared, the OSCC II procedure is as accurate as, and is significantly more precise than, the DMSCC method. The faster sampling rate of the OSCC II at 120/hr has some effect on precision but little effect on accuracy. The method has been adopted as official first action.
开展了一项合作研究，以比较改进后的自动光学体细胞计数程序（OSCC II）与生鲜乳直接显微镜体细胞计数法（DMSCC）。样品用甲醛固定后引入自动分析仪系统。自动进行稀释、澄清和细胞计数。五名合作者参与了该研究；他们以两种不同的采样率对48个样品进行了重复分析。将结果与三名不同分析师报告的DMSCC计数进行比较。统计结果表明，DMSCC方法的标准差为0.0825，而OSCC II在采样率为80/小时时的标准差为0.0434。比较结果时，OSCC II程序与DMSCC方法一样准确，且明显更精确。OSCC II在120/小时的更快采样率对精密度有一定影响，但对准确度影响很小。该方法已被采用为官方首次行动方法。
