Lintner T J, Lange A L, Heald C W, Eberhart R J
The Pennsylvania State University, Department of Veterinary Science and Dairy Science Extension, University Park, Pennsylvania 16802.
J Food Prot. 1987 Feb;50(2):132-135. doi: 10.4315/0362-028X-50.2.132.
Somatic cell count samples (SCCS) for use in comparison of milk somatic cell counting methods were prepared from the cell sediment deposited in a creamery milk separator. Bovine milk somatic cells were resuspended from the sediment, and serial cell dilutions were prepared in bronopol-preserved milk diluent. Over a 1-year period, sets of SCCS were prepared each month and sent to milk-testing laboratories in the U.S.A., Canada and Europe, and counted by the methods in use at those Laboratories: (a) direct microscopic somatic cell count (DMSCC), (b) Fossomatic counter and (c) Coulter counter. Cell counts were normalized to eliminate the effect of month to month variation in the cell content of the SCCS. Counts obtained by the three methods were similar, although Coulter counter results tended to be lower, and significantly lower (P< 0.05) in SCCS with cell counts greater than 700,000 cells/ml than those counts by the other two methods. The effect of shipping on SCCS stability was assessed for SCCS samples sent to and returned from other laboratories, and counted by the Fossomatic method on their return. Counts were similar before and after shipping, except that results for SCCS with cell counts greater than 1,000,000 cells/ml were significantly higher (P<0.05) after their return.
用于比较牛奶体细胞计数方法的体细胞计数样本（SCCS）是由乳品厂牛奶分离器中沉积的细胞沉淀物制备而成。从沉淀物中重新悬浮牛乳腺体细胞，并在溴硝醇保存的牛奶稀释剂中制备系列细胞稀释液。在1年的时间里，每月制备SCCS样本集，并将其送往美国、加拿大和欧洲的牛奶检测实验室，按照这些实验室使用的方法进行计数：（a）直接显微镜体细胞计数（DMSCC），（b）Fossomatic计数器，以及（c）库尔特计数器。对细胞计数进行标准化处理，以消除SCCS细胞含量逐月变化的影响。尽管库尔特计数器的结果往往较低，且在细胞计数大于700,000个细胞/毫升的SCCS中，其结果显著低于（P<0.05）其他两种方法的计数结果，但三种方法获得的细胞计数结果相似。对送往其他实验室并返回的SCCS样本，评估了运输对SCCS稳定性的影响，并在返回时用Fossomatic方法进行计数。运输前后的计数结果相似，只是细胞计数大于1,000,000个细胞/毫升的SCCS返回后的结果显著更高（P<0.05）。
