Lintner T J, Heald C W, Eberhart R J
The Pennsylvania State University, Department of Veterinary Science and Dairy Science Extension, University Park, Pennsylvania 16802.
J Food Prot. 1984 Sep;47(9):694-696. doi: 10.4315/0362-028X-47.9.694.
Somatic cell count samples (SCCS) for use in calibration of milk somatic cell counting methods were prepared from raw bulk milk preserved with potassium dichromate. Somatic cells were separated by centrifugation, then appropriate cell dilutions were prepared in the dichromate-preserved skim milk. Somatic cell counts from SCCS stored at 4°C were stable over a 23-wk period. No bacterial contamination was detected in these samples. In a collaborative study among eight laboratories, SCCS were not affected by usual conditions by shipping. The SCCS can be used as reference standards for the direct microscopic somatic cell count and the Fossomatic and Coulter Counter somatic cell counting methods.
用于校准牛奶体细胞计数方法的体细胞计数样本（SCCS）由用重铬酸钾保存的生鲜混合乳制备而成。通过离心分离体细胞，然后在重铬酸钾保存的脱脂乳中制备适当的细胞稀释液。保存在4°C的SCCS中的体细胞计数在23周内保持稳定。这些样本未检测到细菌污染。在八项实验室参与的协作研究中，SCCS不受运输常规条件的影响。SCCS可用作直接显微镜体细胞计数以及Fossomatic和库尔特计数器体细胞计数方法的参考标准。
