School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China; Key Laboratory of Anhui Ecological Engineering and Biotechnology, Hefei 230601, Anhui, PR China.
School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
Carbohydr Polym. 2019 Apr 1;209:363-371. doi: 10.1016/j.carbpol.2019.01.044. Epub 2019 Jan 14.
In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb. by DEAE sepharose FF and Superdex 75 gel chromatography. The average molecular weight (Mw) of EFSP-1 was 8.75 kDa. Monosaccharides composition analysis indicated that EFSP-1 was a glucan. The structure of EFSP-1 was characterized by analysis of FT-IR, GC-MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp. Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells. The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells. Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.
在这项研究中，从芡种子中提取了一种多糖级分（EFSP-1），通过 DEAE sepharose FF 和 Superdex 75 凝胶层析法获得。EFSP-1 的平均分子量（Mw）为 8.75 kDa。单糖组成分析表明，EFSP-1 是一种葡聚糖。EFSP-1 的结构通过 FT-IR、GC-MS 和 NMR 分析进行了表征，结果表明 EFSP-1 的主链主要由（1→4）-α-D-Glcp 组成，在 O-6 处有支链取代，末端为 T-α-D-Glcp。此外，通过建立胰岛素抵抗 HepG2 和 3T3-L1 细胞研究了 EFSP-1 的降血糖作用。结果表明，EFSP-1 通过激活 IR 细胞中的 PI3K/Akt 信号通路，上调 GLUT-4 的表达，增加葡萄糖的消耗。因此，EFSP-1 可作为改善胰岛素抵抗的潜在功能性食品，用于糖尿病治疗。
