State Key Laboratory of Medicinal Chemistry Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin, 300350, People's Republic of China.
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, People's Republic of China.
Carbohydr Polym. 2021 Aug 1;265:118080. doi: 10.1016/j.carbpol.2021.118080. Epub 2021 Apr 15.
A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata. The absolute molecular weight was determined as 1.26 × 10 g/mol. Monosaccharide composition analysis showed that RLP50-2 mainly consisted of glucose, arabinose, and galactose. Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose. Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models. Moreover, RLP50-2 showed significantly antitumor activities by inhibiting tumor cell proliferation and migration and blocking angiogenesis. These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
从刺梨果实中提取并纯化出一种中性水溶性多糖（RLP50-2）。其重均分子量为 1.26×10^5g/mol。单糖组成分析表明，RLP50-2 主要由葡萄糖、阿拉伯糖和半乳糖组成。结构分析表明，RLP50-2 由→5)-α-L-Araf-(1→、→2,5)-α-L-Araf-(1→、→3,5)-α-L-Araf-(1→、→4)-α-D-Glcp-(1→、→6)-α-D-Glcp-(1→、→3,6)-β-D-Glcp-(1→、→4)-α-D-Galp-(1→、→6)-β-D-Galp-(1→、→2)-β-D-Xylp-(1→、末端α-L-阿拉伯糖和末端β-D-甘露糖组成。细胞和斑马鱼模型的生物测定表明，RLP50-2 具有免疫调节活性。此外，RLP50-2 通过抑制肿瘤细胞增殖和迁移以及阻断血管生成，显示出显著的抗肿瘤活性。这些结果表明，RLP50-2 可作为一种有潜力的免疫调节剂或抗肿瘤候选药物在生物医药领域得到开发。
