Pramita Ayu Dewi, Kristanti Alfinda Novi, Utami Edy Setiti Wida, Manuhara Yosephine Sri Wulan
Laboratory of Plant Tissue Culture, Biology Department, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia.
Laboratory of Organic Chemistry, Chemistry Department, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia.
J Genet Eng Biotechnol. 2018 Dec;16(2):639-643. doi: 10.1016/j.jgeb.2018.05.007. Epub 2018 Jul 5.
(Lour.) Merris one of medicinal plant which was carried out used as antioxidant, anticancer, anti-inflammatory, hepatoprotective, and antimicrobial. Many strategies were used to increase the production of biomass and valuable compounds. This study was to investigate the variation effect of growth regulators and immersion frequency on production of biomass and flavonoid contained of shoots culture in temporary immersion bioreactor. Stem nodes were used as an explants and induction of shoots were done in solid MS medium supplemented with many kinds of growth regulator. The best treatments were used to produce biomass and flavonoid compounds in temporary immersion bioreactor; there are combination of IAA 2 mg/L and BA 4, 6, 8 mg/L and immersion frequency (5 min each 3 h; 15 min each 12 h). Results showed that the growths of shoots in solid MS medium were influenced by supplementation of growth regulators. MS medium supplemented with single cytokinine (6 mg/L kinetin) and combination of auxin (IAA) and cytokinine (BA) caused increasing of shoots growth. Production of biomass of in temporary immersion bioreactor was achieved in long immersion interval (12 h) and highest flavonoid production was obtained in combination treatment of immersion frequency 15 min each 12 h and MS medium supplemented with IAA 2 mg/L, BA 8 mg/L.
（卢尔.）梅里斯是一种药用植物，具有抗氧化、抗癌、抗炎、保肝和抗菌作用。人们采用了多种策略来提高生物量和有价值化合物的产量。本研究旨在探讨生长调节剂和浸没频率对临时浸没式生物反应器中茎段培养物生物量和黄酮类化合物产量的影响。茎节用作外植体，在添加多种生长调节剂的固体MS培养基中诱导芽的生长。最佳处理用于在临时浸没式生物反应器中生产生物量和黄酮类化合物；它们是2mg/L IAA与4、6、8mg/L BA的组合以及浸没频率（每3小时5分钟；每12小时15分钟）。结果表明，固体MS培养基中芽的生长受生长调节剂添加的影响。添加单一细胞分裂素（6mg/L激动素）以及生长素（IAA）和细胞分裂素（BA）组合的MS培养基可促进芽的生长。在长浸没间隔（12小时）下可实现临时浸没式生物反应器中生物量的生产，在浸没频率为每12小时15分钟且MS培养基添加2mg/L IAA、8mg/L BA的组合处理中获得最高的黄酮类化合物产量。
