Lim Hong Kiat, O'Neill Helen C
Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University, Gold Coast, QLD, Australia.
Research School of Biology, Australian National University, Canberra, ACT, Australia.
Front Cell Dev Biol. 2019 Jan 24;7:1. doi: 10.3389/fcell.2019.00001. eCollection 2019.
Stromal cells in spleen organize tissue into red pulp, white pulp and marginal zone, and also interact with hematopoietic cells to regulate immune responses. This study has used phenotypic information of a previously described spleen stromal cell line called 5G3, which supports restricted hematopoiesis , to identify an equivalent stromal cell subset and to test its capacity to support hematopoiesis. Using stromal cell fractionation, phenotypic analysis, as well as cell growth and hematopoietic support assays, the Sca-1gp38Thy1.2CD29CD51 fraction of spleen stroma has been identified as an equivalent stromal subset resembling the 5G3 cell counterpart. While heterogeneity may still exist within that subset, it has been shown to have superior hematopoietic support capacity compared with the 5G3 cell line, and all other spleen stromal cell fractions tested.
脾脏中的基质细胞将组织组织成红髓、白髓和边缘区，还与造血细胞相互作用以调节免疫反应。本研究利用了一种先前描述的名为5G3的脾基质细胞系的表型信息，该细胞系支持有限的造血作用，以鉴定等效的基质细胞亚群并测试其支持造血的能力。通过基质细胞分级分离、表型分析以及细胞生长和造血支持测定，已确定脾基质的Sca-1gp38Thy1.2CD29CD51部分为类似于5G3细胞对应物的等效基质亚群。虽然该亚群内可能仍存在异质性，但已证明其与5G3细胞系以及测试的所有其他脾基质细胞部分相比，具有更强的造血支持能力。
