整体转录组空间分析与蛋白质标记物的整合。
Integration of whole transcriptome spatial profiling with protein markers.
机构信息
Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA.
New York Genome Center, New York, NY, USA.
出版信息
Nat Biotechnol. 2023 Jun;41(6):788-793. doi: 10.1038/s41587-022-01536-3. Epub 2023 Jan 2.
Abstract
Spatial transcriptomics and proteomics provide complementary information that independently transformed our understanding of complex biological processes. However, experimental integration of these modalities is limited. To overcome this, we developed Spatial PrOtein and Transcriptome Sequencing (SPOTS) for high-throughput simultaneous spatial transcriptomics and protein profiling. Compared with unimodal measurements, SPOTS substantially improves signal resolution and cell clustering and enhances the discovery power in differential gene expression analysis across tissue regions.
摘要
空间转录组学和蛋白质组学提供了互补的信息，它们独立地改变了我们对复杂生物过程的理解。然而，这些模式的实验整合是有限的。为了克服这一问题，我们开发了高通量同时进行空间转录组学和蛋白质谱分析的空间蛋白质和转录组测序（SPOTS）技术。与单模态测量相比，SPOTS 大大提高了信号分辨率和细胞聚类，并增强了在组织区域的差异基因表达分析中发现差异的能力。
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