[免疫荧光法与免疫酶法在快速诊断鼻咽分泌物中呼吸道合胞病毒的比较]

[Comparison between immunofluorescence and immunoenzymatic assay for the rapid diagnosis of the respiratory syncytial virus in nasopharyngeal secretions].

作者信息

Chiparelli H, Russi J C, Martorell E, Arbiza J R, Cánepa E, Hortal M

机构信息

Departamento de Laboratorios, Ministerio de Salud Pública, Montevideo, Uruguay.

出版信息

Rev Argent Microbiol. 1988 Oct-Dec;20(4):201-4.

PMID:3073406

Abstract

An enzyme immunoassay, RSV-EIA Abbot, was evaluated by comparison with indirect immunofluorescence. Nasopharyngeal secretions obtained from 95 infants and young children with acute respiratory infections were examined for the presence of respiratory syncytial virus antigens with both methods. Specimens were stored at -70 degrees C before being tested by EIA. Out of 60 samples positive by indirect immunofluorescence, 46 were also positive by RSV-EIA (sensitivity 78.7%) and 34 out of 35 immunofluorescence negative specimens were negative by RSV-EIA (specificity 97.1%). Therefore, the EIA appears to be an acceptable test for the rapid detection of RSV as an alternative for indirect immunofluorescence.

摘要

通过与间接免疫荧光法进行比较，对一种酶免疫测定法（呼吸道合胞病毒酶免疫测定法，雅培公司产品）进行了评估。采用这两种方法对95例患有急性呼吸道感染的婴幼儿的鼻咽分泌物进行检测，以确定是否存在呼吸道合胞病毒抗原。在通过酶免疫测定法检测之前，样本保存在-70摄氏度。在60例经间接免疫荧光法检测呈阳性的样本中，有46例经呼吸道合胞病毒酶免疫测定法检测也呈阳性（灵敏度78.7%）；在35例免疫荧光法检测呈阴性的样本中，有34例经呼吸道合胞病毒酶免疫测定法检测也呈阴性（特异性97.1%）。因此，酶免疫测定法似乎是一种可接受的用于快速检测呼吸道合胞病毒的检测方法，可作为间接免疫荧光法的替代方法。