Wei Yuli, Cao Junwei, Yao Huimin, Mao Haiyan, Zhu Kelei, Li Meng, Liu Rulong, Fang Jiasong
Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, PR China.
State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Wuhan 430074, PR China.
Int J Syst Evol Microbiol. 2019 Apr;69(4):1035-1040. doi: 10.1099/ijsem.0.003265. Epub 2019 Feb 8.
A novel marine Gram-stain-negative, non-spore-forming, motile, aerobic, coccoid or ovoid bacterium, designated as strain DSL-16, was isolated from a tidal flat sediment on the East China Sea and characterized phylogenetically and phenotypically. Optimal growth of the strain occurred at 35 °C (range 4-40 °C), at pH 6 (range 5-11) and with 4 % (w/v) NaCl (range 1-14 %). The nearest phylogenetic neighbour was Paracoccusseriniphilus DSM 14827 (98.2 % 16S rRNA gene sequence similarity). The digital DNA-DNA hybridization value between strain DSL-16 and P. seriniphilus DSM 14827 was 19.5±2.2 %. The average nucleotide identity value between strain DSL-16 and P. seriniphilus DSM 14827 was 83.6 %. The sole respiratory ubiquinone was Q-10. The major polar lipids were phosphatidylmonomethylethanolamine (PME), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), diphosphatidyglycerol (DPG) and glycolipid (GL). The predominant cellular fatty acids of strain DSL-16 were C18 : 1ω7c, C18 : 0 and 11-methyl C18 : 1ω7c. The G+C content of the genomic DNA was 64.5 mol%. The combined genotypic and phenotypic data indicated that strain DSL-16 represents a novel species of the genus Paracoccus, for which the name Paracoccus sediminilitoris sp. nov. is proposed. The type strain is DSL-16 (=KCTC 62644=MCCC 1K03534).
从中国东海潮滩沉积物中分离出一株新型海洋革兰氏染色阴性、无芽孢、运动性好、需氧的球状或卵形细菌，命名为DSL-16菌株，并对其进行了系统发育和表型特征分析。该菌株的最适生长温度为35 °C（范围4-40 °C），最适pH为6（范围5-11），最适NaCl浓度为4%（w/v）（范围1-14%）。系统发育关系最近的邻接菌株是嗜血清副球菌DSM 14827（16S rRNA基因序列相似性为98.2%）。DSL-16菌株与嗜血清副球菌DSM 14827之间的数字DNA-DNA杂交值为19.5±2.2%。DSL-16菌株与嗜血清副球菌DSM 14827之间的平均核苷酸同一性值为83.6%。唯一的呼吸醌为Q-10。主要极性脂为磷脂酰单甲基乙醇胺（PME）、磷脂酰甘油（PG）、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、二磷脂酰甘油（DPG）和糖脂（GL）。DSL-16菌株的主要细胞脂肪酸为C18 : 1ω7c、C18 : 0和11-甲基C18 : 1ω7c。基因组DNA的G+C含量为64.5 mol%。综合基因型和表型数据表明，DSL-16菌株代表副球菌属的一个新物种，为此提出了新名称嗜潮滩副球菌（Paracoccus sediminilitoris sp. nov.）。模式菌株为DSL-16（=KCTC 62644=MCCC 1K03534）。
