Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano-IT, Italy.
Department of Materials Science, University of Milano-Bicocca, via R. Cozzi 55, 20125 Milano-IT, Italy.
Molecules. 2019 Feb 7;24(3):589. doi: 10.3390/molecules24030589.
Gelatin is a biopolymer with interesting properties that can be useful for biomaterial design for different applications such as drug delivery systems, or 3D scaffolds for tissue engineering. However, gelatin suffers from poor mechanical stability at physiological temperature, hence methods for improving its properties are highly desirable. In the present work, a new chemical cross-linking strategy based on triazolinedione ene-type chemistry towards stable hydrogel is proposed. Two different homobifunctional 1,2,4-triazoline-3,5(4)-diones, namely 4,4'-hexane-1,6-diylbis(3-1,2,4-triazoline-3,5(4)-dione) and 4,4'-methylenebis(4,1-phenylene) were used as cross-linkers in different ratio to tyrosine residues in gelatin. The reaction was proved effective in all experimented conditions and hydrogels featured with different thermal stability were obtained. In general, the higher the cross-linker/tyrosine ratio, the more thermostable the hydrogel. The swelling properties are strictly dependent upon the chemical nature of the cross-linker.
明胶是一种具有有趣性质的生物聚合物，可用于设计不同应用的生物材料，如药物传递系统或组织工程的 3D 支架。然而，明胶在生理温度下的机械稳定性较差，因此需要改进其性能的方法。在本工作中，提出了一种基于三唑啉二酮烯型化学的新的化学交联策略，以获得稳定的水凝胶。使用两种不同的同双官能团 1,2,4-三唑啉-3,5(4)-二酮，即 4,4'-己烷-1,6-二基双（3-1,2,4-三唑啉-3,5(4)-二酮）和 4,4'-[亚甲基双（4,1-亚苯基）]（3-1,2,4-三唑啉-3,5(4)-二酮），以不同的比例作为交联剂与明胶中的酪氨酸残基反应。证明该反应在所有实验条件下均有效，并获得了具有不同热稳定性的水凝胶。一般来说，交联剂/酪氨酸的比例越高，水凝胶的热稳定性越高。溶胀性能严格取决于交联剂的化学性质。
