Dept. of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, 20126 Milano, Italy.
Dept. of Physics "Giuseppe Occhialini", University of Milano-Bicocca, P.zza della Scienza 3, 20126 Milano, Italy.
Langmuir. 2021 Dec 7;37(48):14050-14058. doi: 10.1021/acs.langmuir.1c02080. Epub 2021 Nov 22.
Hydrogels are useful platforms as three-dimensional (3D) scaffolds for cell culture, drug-release systems, and regenerative medicine applications. Here, we propose a novel chemical cross-linking approach by the use of 3,4-diethoxy-3-cyclobutene-1,2-dione or diethyl squarate for the preparation of 5 and 10% w/v gelatin-based hydrogels. Hydrogels showed good swelling properties, and the 5% gelatin-based hydrogel proved suitable as a 3D cell culture scaffold for the chondrocyte cell line C28/I2. In addition, diffusion properties of different sized molecules inside the hydrogel were determined.
水凝胶是细胞培养、药物释放系统和再生医学应用的三维（3D）支架的有用平台。在这里，我们提出了一种新的化学交联方法，使用 3,4-二乙氧基-3-环丁烯-1,2-二酮或二乙基丙二酸盐来制备 5%和 10% w/v 的明胶基水凝胶。水凝胶表现出良好的溶胀性能，5%明胶基水凝胶可作为软骨细胞系 C28/I2 的 3D 细胞培养支架。此外，还确定了不同大小分子在水凝胶内的扩散性质。
