非洲爪蟾单倍体、三倍体和杂交胚胎的产生。

Generation of Xenopus Haploid, Triploid, and Hybrid Embryos.

作者信息

Gibeaux Romain, Heald Rebecca

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

出版信息

Methods Mol Biol. 2019;1920:303-315. doi: 10.1007/978-1-4939-9009-2_18.

DOI:10.1007/978-1-4939-9009-2_18

PMID:30737699

Abstract

Frog species of the genus Xenopus are widely used for studies of cell and developmental biology, and recent genome sequencing has revealed interesting phylogenetic relationships. Here we describe methods to generate haploid, triploid, and hybrid species starting from eggs and sperm of Xenopus laevis and Xenopus tropicalis that enable investigation of how genome size and content affect physiology at the organismal, cellular, and subcellular levels.

摘要

非洲爪蟾属的蛙类被广泛用于细胞和发育生物学研究，最近的基因组测序揭示了有趣的系统发育关系。在这里，我们描述了从非洲爪蟾和热带爪蟾的卵子和精子出发，生成单倍体、三倍体和杂交物种的方法，这些方法能够研究基因组大小和内容如何在生物体、细胞和亚细胞水平上影响生理学。