Lin Danqiu, Zhu Sidong, Chen Yong, Huang Yizhe, Yang Jifang, Chen Jigang
College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 Jun;112(6):927-933. doi: 10.1007/s10482-019-01226-2. Epub 2019 Feb 8.
Strain IO390502, isolated from surface seawater in the Indian Ocean, was characterised using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain IO390502 belongs to the genus Paracoccus and is closely related to Paracoccus seriniphilus DSM 14827 (97.6%), followed by P. zeaxanthinifaciens JCM 21774 (97.5%), P. homiensis DSM 17862 (97.3%), P. marcusii DSM 11574 (97.2%), P. haeundaensis BC 74171 (97.0%) and P. carotinifaciens E-396 (97.0%). Cells are Gram-negative, aerobic, poly-β-hydroxybutyrate-accumulating, motile, rod-shaped, and forms creamy-white colonies. Optimal growth occurred at 25-30 °C, pH 5-8, and in the presence of 3-8% NaCl. The genome of strain IO390502 has a G+C content of 64.9 mol% and a 3.5 Mb chromosome. The in silico DNA-DNA hybridisation and average nucleotide identity values between strain IO390502 and the three closely related taxa, P. seriniphilus DSM 14827, P. zeaxanthinifaciens JCM 21774 and P. homiensis DSM 17862, are 19.6%, 21.9% and 20.6%, and 76.0%, 79.9% and 77.8%, respectively. Phosphatidylglycerol is the major lipid present, ubiquinone-10 (Q-10) is the sole isoprenoid quinone, and the major cellular fatty acid is Cω7c. Based on data from phenotypic tests and genotypic differences between strain IO390502 and its close phylogenetic relatives, strain IO390502 represents a new species belonging to the genus Paracoccus, for which the name Paracoccus indicus sp. nov. is proposed. The type strain is IO390502 (= JCM 32637 = CCTCC AB 2018071).
菌株IO390502是从印度洋表层海水中分离得到的，采用多相分类法对其进行了表征。基于16S rRNA基因序列的系统发育分析表明，菌株IO390502属于副球菌属，与嗜丝氨酸副球菌DSM 14827密切相关（97.6%），其次是玉米黄质副球菌JCM 21774（97.5%）、霍米副球菌DSM 17862（97.3%）、马库斯副球菌DSM 11574（97.2%）、海州湾副球菌BC 74171（97.0%）和胡萝卜素副球菌E-396（97.0%）。细胞为革兰氏阴性、需氧、能积累聚-β-羟基丁酸、运动、杆状，形成乳脂白色菌落。在25-30°C、pH 5-8和3-8% NaCl存在的条件下生长最佳。菌株IO390502的基因组G+C含量为64.9 mol%，有一条3.5 Mb的染色体。菌株IO390502与三个密切相关的分类单元嗜丝氨酸副球菌DSM 14827、玉米黄质副球菌JCM 21774和霍米副球菌DSM 17862之间的电子DNA-DNA杂交和平均核苷酸同一性值分别为19.6%、21.9%和20.6%，以及76.0%、79.9%和77.8%。磷脂酰甘油是主要的脂质成分，泛醌-10（Q-10）是唯一的类异戊二烯醌，主要的细胞脂肪酸是Cω7c。基于菌株IO390502及其近缘系统发育亲缘种的表型测试数据和基因型差异，菌株IO390502代表副球菌属的一个新物种，为此提出了印度副球菌新种的名称。模式菌株为IO390502（=JCM 32637=CCTCC AB 2018071）。
