Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236, Łódź, Poland.
Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100, Puławy, Poland.
Food Chem Toxicol. 2019 Mar;125:614-620. doi: 10.1016/j.fct.2019.02.014. Epub 2019 Feb 6.
The objective of this study is to investigate the biological effects of phenolic compounds extracted from the sea buckthorn berries on oxidative stress and hemostasis. The sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) berries are rich in flavonoids and non-polar compounds. In this study, the activity of the phenolic fraction from the sea buckthorn berries was evaluated in vitro in comparison with three phenolic compounds: isorhamnetin (compound 1) and its two new derivatives: compound 2 (isorhamnetin 3-O-beta-glucoside-7-O-alfa-rhamnoside) and compound 3 (isorhamnetin 3-O-beta-glucoside-7-O-alfa-(3"'-isovaleryl)-rhamnoside). The impact of these phenolic compounds and the phenolic fraction against the effect of the donor of hydroxyl radicals - HO/Fe on proteins and lipids in human plasma was measured. Additionally, the aim of the study was to determine the effect of these phenolic compounds and the phenolic fraction on various typical hemostasis parameters. Our results show that the used derivatives of isorhamnetin possess different biological properties (e.g. antioxidant, anti-platelet and anticoagulant). The tested compounds can be seen as new natural beneficial compounds to be used in prevention and treatment of cardiovascular diseases.
本研究旨在探讨沙棘浆果中提取的酚类化合物对氧化应激和止血的生物学影响。沙棘（Elaeagnus rhamnoides (L.) A. Nelson）浆果富含类黄酮和非极性化合物。在这项研究中，我们评估了沙棘浆果酚类部位的体外活性，并与三种酚类化合物进行了比较：异鼠李素（化合物 1）及其两个新衍生物：化合物 2（异鼠李素 3-O-β-葡萄糖苷-7-O-α-鼠李糖苷）和化合物 3（异鼠李素 3-O-β-葡萄糖苷-7-O-α-（3“-异戊酰基）-鼠李糖苷）。测量了这些酚类化合物和酚类部位对人血浆中羟自由基供体 HO/Fe 对蛋白质和脂质的影响。此外，本研究的目的还在于确定这些酚类化合物和酚类部位对各种典型止血参数的影响。我们的结果表明，所使用的异鼠李素衍生物具有不同的生物学特性（例如抗氧化、抗血小板和抗凝血）。这些测试的化合物可以作为预防和治疗心血管疾病的新的天然有益化合物。
