Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation - State Research Institute, ul. Czartoryskich 8, 24-100 Puławy, Poland.
Laboratory of Cytogenetics, Faculty of Biology and Environmental Protection, University of Łódź, ul. Banacha 12/16, 90 237 Łódź, Poland.
Phytochemistry. 2019 Jul;163:178-186. doi: 10.1016/j.phytochem.2019.03.001. Epub 2019 Apr 3.
Eight undescribed isorhamnetin glycosides, acylated with isovaleric acid were isolated from the fruit of sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson). Structures of the purified compounds were determined using one and two-dimensional NMR spectroscopy, mass spectrometry and chemical methods. In addition, the cytotoxic activity of the phenolic-rich fraction of sea buckthorn fruit and its major flavonoids against colon cell lines, HT-29, HCT-116 and Caco-2, was determined. While the phenolic fraction was moderately active against HT-29 and HCT-116, all investigated purified flavonoids showed significantly weaker activity. This is most probably the first report about isorhamnetin glycosides acylated with isovaleric acid.
从沙棘（Elaeagnus rhamnoides（L.）A. Nelson）的果实中分离得到了八种未被描述的异鼠李素糖苷，它们都被异戊酸酰化。使用一维和二维 NMR 光谱、质谱和化学方法确定了纯化化合物的结构。此外，还测定了沙棘果实中富含酚类物质及其主要类黄酮对结肠细胞系 HT-29、HCT-116 和 Caco-2 的细胞毒性活性。虽然酚类部分对 HT-29 和 HCT-116 具有中等活性，但所有研究的纯化类黄酮的活性明显较弱。这很可能是关于异鼠李素糖苷被异戊酸酰化的第一个报道。
