Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Shanghai 201306, China.
Int J Biol Macromol. 2019 May 15;129:377-385. doi: 10.1016/j.ijbiomac.2019.02.043. Epub 2019 Feb 8.
In the present study, crude polysaccharides were extracted from Gelidium pacificum Okamura, and further purified to obtain the sulfated polysaccharide with molecular weight of 28,807 Da. Its monosaccharide composition mainly consisted of xylose (7.1%), galactose (59.7%) and galacturonic acid (19.76%). And the sulfate ester content of the sulfated polysaccharide was estimated as 8.8%. Structure analysis showed that the sulfated polysaccharide comprised of 1,4-linked-α-D-Galp3S, 1,2-linked-α-D-Xylp and 1,3-linked-β-D-GalpA residues, respectively. Its anti-inflammatory effects were investigated in LPS-stimulated human monocytic (THP-1) cells. The sulfated polysaccharide at a concentration of 5 μg/mL fully protected the THP-1 cells against LPS-stimulated cytotoxicity. Furthermore, the addition of sulfated polysaccharide resulted in a significant reduction of NO production in LPS-treated cells, and this effect appeared to be dose-related. The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells. These results showed the sulfated polysaccharide not only provided a good protection against LPS-induced cell toxicity, but also exerted an anti-inflammatory effect via the TLR4 signaling pathway.
在本研究中，从冈村海萝中提取了粗多糖，并进一步纯化得到了分子量为 28807 Da 的硫酸多糖。其单糖组成主要由木糖（7.1%）、半乳糖（59.7%）和半乳糖醛酸（19.76%）组成。硫酸多糖的硫酸酯含量估计为 8.8%。结构分析表明，硫酸多糖由 1,4-连接的-α-D-Galp3S、1,2-连接的-α-D-Xylp 和 1,3-连接的-β-D-GalpA 残基组成。在 LPS 刺激的人单核细胞（THP-1）细胞中研究了其抗炎作用。硫酸多糖在 5 μg/mL 的浓度下完全保护 THP-1 细胞免受 LPS 刺激的细胞毒性。此外，添加硫酸多糖可显著减少 LPS 处理细胞中的 NO 产生，且这种作用呈剂量依赖性。硫酸多糖（5 μg/mL）可显著抑制 LPS 刺激的 THP-1 细胞中 TLR-4、髓样分化因子（MyD88）和肿瘤坏死因子受体相关因子-6（TRAF-6）的 mRNA 和蛋白表达。这些结果表明，硫酸多糖不仅对 LPS 诱导的细胞毒性提供了良好的保护作用，而且还通过 TLR4 信号通路发挥了抗炎作用。
