从盐生植物白刺果实中提取水溶性多糖的结构表征及其抗氧化和降血脂活性。
Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities.
机构信息
Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112 Gafsa, Tunisia.
Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia; Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France.
出版信息
Int J Biol Macromol. 2019 May 15;129:422-432. doi: 10.1016/j.ijbiomac.2019.02.049. Epub 2019 Feb 8.
The structure, antioxidant and anti-hyperlipidemic activities of polysaccharides from Nitraria retusa fruits (named as NRFP) were investigated. The NRFP fraction, with a molecular weight of 66.5 kDa, was composed of a β-(1 → 3)-glucan, containing neutral sugars (69.1%) but also uronic acids up to 23.1% due to pectin structure. The monosaccharide composition highlighted a polymer composed of glucose (41.4%), galacturonic acid (30.5%), galactose (12.6%), arabinose (11.8%) and rhamnose (3.70%). In the antioxidant assays, NRFP exhibited effective total antioxidant capacity (IC = 7.82 mg/ml), scavenging activities on DPPH radical (IC = 0.87 mg/ml) and hydrogen peroxide (IC = 2.03 mg/ml). In addition, NRFP proved protective effects on HO induced hemolysis (IC = 66.2 μg/ml). In vivo NRFP reduced the hyperlipidemia, hepatotoxicity, cardiovascular and coronary diseases induced by Triton X-100.
研究了新疆盐生植物白刺果多糖（NRFP）的结构、抗氧化和抗高血脂活性。NRFP 级分的分子量为 66.5 kDa，由β-（1→3）-葡聚糖组成，含有中性糖（69.1%），但由于果胶结构，也含有高达 23.1%的糖醛酸。单糖组成突出了一种由葡萄糖（41.4%）、半乳糖醛酸（30.5%）、半乳糖（12.6%）、阿拉伯糖（11.8%）和鼠李糖（3.70%）组成的聚合物。在抗氧化试验中，NRFP 表现出有效的总抗氧化能力（IC=7.82 mg/ml）、对 DPPH 自由基（IC=0.87 mg/ml）和过氧化氢（IC=2.03 mg/ml）的清除活性。此外，NRFP 对 HO 诱导的溶血具有保护作用（IC=66.2 μg/ml）。体内试验结果表明，NRFP 能降低 Triton X-100 诱导的高血脂、肝毒性、心血管疾病和冠心病。
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