竹（Phyllostachys pubescens Mazel）叶水溶性多糖的结构表征、抗氧化和抗菌活性。
Structural characterization, antioxidant and antimicrobial activity of water-soluble polysaccharides from bamboo (Phyllostachys pubescens Mazel) leaves.
机构信息
Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Zhejiang University of Science and Technology, Hangzhou 310023, PR China; College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Zhejiang University of Science and Technology, Hangzhou 310023, PR China; College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
出版信息
Int J Biol Macromol. 2020 Jan 1;142:432-442. doi: 10.1016/j.ijbiomac.2019.09.115. Epub 2019 Oct 5.
One water-soluble neutral polysaccharides (NPs), with an average molecular weight of 5.77 × 10 Da, was isolated from bamboo (Phyllostachys pubescens Mazel) leaves and the yield was 10.2% based on weight of raw material. Successively, two fractions (NPs-A and NPs-B) could be obtained after being eluted by DEAE-Sepharose. Structural analysis indicated that NPs was formed by 1,4-β-linked xylp backbone. Monosaccharide composition and methylation analysis suggested that glucose, arabinose, galactose and rhamnose constituted side chains with the molar ratio of 1.22:1.79:1.89:4.46 with the glycosidic linkages of →4)-Glc-(1→, →3,5)-Ara-(1→, →4)-Gla-(1→, →4)-Rha-(1 → and → 2,4)-Rha-(1→. Antioxidant assays demonstrated that NPs exhibited relatively high activity in a dose-dependent manner with the highest 85.9% DPPH and 99.78% ABTS free radical scavenging rate when the concentration was 4.0 and 3.0 mg/mL, respectively. Moreover, NPs exhibited obvious growth inhibitory against E. coli, S. aureus and B. subtilis when the NPs concentration was in range of 0.50-50.0 mg/mL. Therefore, we concluded that NPs from bamboo leaves could be used as a potential and natural bacteriostat and antioxidant.
一种水溶性中性多糖（NPs），平均分子量为 5.77×10 Da，从竹叶中分离得到，产率为 10.2%（以原料重量计）。然后，用 DEAE-Sepharose 洗脱后可得到两种级分（NPs-A 和 NPs-B）。结构分析表明，NPs 由 1,4-β-连接的木糖主链组成。单糖组成和甲基化分析表明，葡萄糖、阿拉伯糖、半乳糖和鼠李糖构成侧链，摩尔比为 1.22:1.79:1.89:4.46，糖苷键连接方式为→4)-Glc-(1→、→3,5)-Ara-(1→、→4)-Gla-(1→、→4)-Rha-(1→和→2,4)-Rha-(1→。抗氧化试验表明，NPs 具有较高的抗氧化活性，在 4.0 和 3.0 mg/mL 时，DPPH 和 ABTS 自由基清除率最高，分别为 85.9%和 99.78%。此外，NPs 对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的生长抑制作用明显，当 NPs 浓度在 0.50-50.0 mg/mL 范围内时。因此，我们得出结论，竹叶中的 NPs 可以作为一种潜在的天然抑菌剂和抗氧化剂。
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