Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
Methods. 2019 Jun 1;162-163:23-30. doi: 10.1016/j.ymeth.2019.02.004. Epub 2019 Feb 8.
Chloramphenicol is a broad-spectrum antibiotic targeting the protein synthesis machinery by binding to the bacterial ribosome. Chloramphenicol has been considered a classic general inhibitor of translation, blocking the accommodation of aa-tRNA into the A site of the large ribosomal subunit. However, recent studies suggest that this proposed mechanism is a simplification and that the effect of chloramphenicol on mRNA translation is much more dynamic. By tracking single dye-labelled elongator and initiator tRNAs in Escherichia coli cells treated with chloramphenicol, we observe the direct effect of chloramphenicol on translation kinetics. We find clear indications of slow but significant mRNA translation on drug bound ribosomes.
氯霉素是一种广谱抗生素，通过与细菌核糖体结合来靶向蛋白质合成机制。氯霉素一直被认为是一种经典的通用翻译抑制剂，可阻止氨酰基-tRNA 进入大亚基核糖体的 A 位。然而，最近的研究表明，这种提出的机制过于简化，氯霉素对 mRNA 翻译的影响要复杂得多。通过在氯霉素处理的大肠杆菌细胞中跟踪单个荧光标记的延伸因子和起始因子 tRNA，我们观察到氯霉素对翻译动力学的直接影响。我们发现药物结合的核糖体上 mRNA 翻译速度缓慢但显著的明确迹象。
