Maruyama Tsuyoshi E, Hosoi Yoshihisa
Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Tsukuba, Japan.
Front Plant Sci. 2019 Jan 28;10:31. doi: 10.3389/fpls.2019.00031. eCollection 2019.
Somatic embryogenesis (SE) in not only one of the most promising techniques for mass propagation of selected trees, but also is a valuable tool for basic research studies in cell biology and genetic engineering, and it allows the long-term conservation of genetic resources by cryopreservation techniques. This review reports the most recent progress in SE, protoplast culture, and cryopreservation of four important Japanese pines ( var. , and ). Induction of embryogenic tissues (ET), embryogenic culture maintenance/proliferation, production of somatic embryos, germination, and conversion to plants are described focusing on the protocols most commonly reported for plant production in species through to SE.
体细胞胚胎发生不仅是用于选定树木大规模繁殖的最具前景的技术之一，也是细胞生物学和基因工程基础研究的宝贵工具，并且它通过冷冻保存技术实现遗传资源的长期保存。本综述报道了四种重要日本松树（ 变种、 和 ）在体细胞胚胎发生、原生质体培养及冷冻保存方面的最新进展。重点围绕通过体细胞胚胎发生在 物种中最常报道的用于植物生产的方案，描述了胚性组织（ET）的诱导、胚性培养物的维持/增殖、体细胞胚的产生、萌发以及转化为植株的过程。
