Prasad Nanda, Vijay Sarvesh, Reddy A Yashwanth, Nonitha S
Department of Oral and Maxillofacial Pathology, Sri Rajiv Gandhi College of Dental Sciences and Hospital, Bengaluru, Karnataka, India.
Dent Res J (Isfahan). 2019 Jan-Feb;16(1):7-11.
The purpose of this study was to determine the effects of menthol-flavored substances at the cellular level in different mucosal sites of the oral cavity and to compare the cellular changes between individuals without the habit of chewing menthol-flavored substances and individuals with the habit.
This was an experimental cytology study including a total of 500 individuals belonging to the age group of 18-45 years based on the inclusion or exclusion criteria. The selected participants were divided into two groups of 250 participants each, based on participants not having the habit of chewing menthol-flavored substances (Group I) and participants having the habit of chewing menthol-flavored substances (Group II). Cytological smears were taken by gently scraping the mucosal surfaces in different sites of the oral mucosa using a wooden spatula and stained with Papanicolaou, analyzed under microscope for any cellular changes. The results were tabulated and statistically analyzed using Chi-square test and Fisher's exact test. < 0.05 was considered statistically significant.
Micronuclei seen in all the participants belonging to group with the habit of chewing menthol-flavored substances with a < 0.001 which was considered highly significant. Alteration in the nuclear-cytoplasmic ratio was also seen = 0.001, which showed significant at 1% significance level.
Participants with habit of chewing menthol-flavored substances showed the presence of micronuclei and slight alteration in the nuclear-cytoplasmic ratio, which could be directly related to genotoxicity and cell damage.
本研究的目的是确定薄荷醇味物质在口腔不同黏膜部位细胞水平上的作用，并比较没有咀嚼薄荷醇味物质习惯的个体与有该习惯的个体之间的细胞变化。
这是一项实验细胞学研究，根据纳入或排除标准，共纳入500名年龄在18 - 45岁的个体。根据是否有咀嚼薄荷醇味物质的习惯，将选定的参与者分为两组，每组250人，其中没有咀嚼薄荷醇味物质习惯的为第一组，有咀嚼薄荷醇味物质习惯的为第二组。用木质刮勺轻轻刮取口腔黏膜不同部位的黏膜表面制作细胞学涂片，并用巴氏染色法染色，在显微镜下分析是否有细胞变化。将结果制成表格，并使用卡方检验和费舍尔精确检验进行统计分析。P < 0.05被认为具有统计学意义。
在所有有咀嚼薄荷醇味物质习惯的参与者中均观察到微核，P < 0.001，被认为具有高度统计学意义。核质比的改变也被观察到，P = 0.001，在1%显著性水平上显示出显著性。
有咀嚼薄荷醇味物质习惯的参与者表现出微核的存在以及核质比的轻微改变，这可能与遗传毒性和细胞损伤直接相关。
