Section of Experimental Oncology and Nanomedicine, Head and Neck Surgery, Department of Otorhinolaryngology, Else Kröner-Fresenius-Foundation-Professorship, Universitätsklinikum Erlangen, Erlangen, Germany.
Institute of Legal Medicine, Ludwig-Maximilians-Universität München, Munchen, Germany.
Tissue Eng Part A. 2019 Nov;25(21-22):1470-1477. doi: 10.1089/ten.TEA.2019.0009. Epub 2019 May 2.
Losing one's ability to speak, because of tissue deficiency at the vocal fold (VF), leads to serious impairment in the quality of life. Until now, there is no successful approach for regenerating the VF. The aim of this study was to show the advantage of magnetic nanoparticles in the generation of scaffold-free three-dimensional (3D) VF cell constructs by magnetic tissue engineering (MTE). Rabbit VF fibroblasts were used to establish MTE: after cellular uptake of superparamagnetic iron oxide nanoparticles (SPIONs), cells can be controlled with a magnetic field thereby forming solid 3D cell structures. To transfer this method into human cells, SPIONs were adapted accordingly and tested for their influence on human VF (hVF) cells and for their ability to perform MTE with hVF cells. Of interest, the cell number and the magnet's shape influence the form of the rabbit VF cell construct. After successful characterization of hVF cells, biocompatibility analyses revealed no significant influence of SPIONs on them, thus 3D hVF cell constructs could be successfully generated by MTE. These basic results are important to develop MTE as an innovative method to regenerate functional VFs. We expect that studies, including MTE as an elegant, far-field controlled and touchless technology, will translate MTE VF bioconstructs into reconstructive laryngeal medicine. Impact Statement This study aims at nanotechnology for regenerative medicine by magnetic tissue engineering (MTE). New approaches for vocal fold (VF) reconstruction are desperately needed. Superparamagnetic iron oxide nanoparticles offer innovative, scaffold-free potentials for tissue engineering: MTE. By using MTE we could generate functional multilayered human VF cell constructs, which can consequently be used to regenerate the voice in patients with VF injuries.
由于声带（VF）组织缺陷导致丧失说话能力，会严重影响生活质量。到目前为止，还没有成功的方法来再生 VF。本研究旨在展示磁性纳米颗粒在通过磁组织工程（MTE）生成无支架三维（3D）VF 细胞构建体方面的优势。使用兔 VF 成纤维细胞建立 MTE：细胞摄取超顺磁氧化铁纳米颗粒（SPIONs）后，可以通过磁场控制细胞，从而形成固体 3D 细胞结构。为了将这种方法转化为人类细胞，相应地适应了 SPIONs，并对其对人类 VF（hVF）细胞的影响及其与 hVF 细胞进行 MTE 的能力进行了测试。有趣的是，细胞数量和磁铁形状影响兔 VF 细胞构建体的形状。成功表征 hVF 细胞后，生物相容性分析显示 SPIONs 对其没有明显影响，因此可以通过 MTE 成功生成 3D hVF 细胞构建体。这些基础结果对于将 MTE 开发为一种再生功能性 VF 的创新方法非常重要。我们预计，包括 MTE 作为一种优雅、远场控制和非接触式技术的研究，将把 MTE VF 生物构建体转化为重建性喉医学。 影响声明 本研究旨在通过磁组织工程（MTE）实现再生医学中的纳米技术。迫切需要新的声带（VF）重建方法。超顺磁氧化铁纳米颗粒为组织工程提供了创新的、无支架的潜力：MTE。通过使用 MTE，我们可以生成功能性多层人类 VF 细胞构建体，随后可以将其用于再生 VF 损伤患者的声音。
