Jiangsu Key Laboratory of Oral Diseases , Nanjing Medical University , Nanjing , Jiangsu 210029 , China.
Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering , Southeast University , Nanjing , Jiangsu 210096 , China.
ACS Appl Mater Interfaces. 2018 Dec 26;10(51):44279-44289. doi: 10.1021/acsami.8b17427. Epub 2018 Dec 12.
One of the key factors in tissue engineering and regenerative medicine is to optimize the interaction between seed cells and scaffolds such that the cells can grow in naturally biomimetic conditions. Their similarity to macromolecules and many unique properties mean that functional nanoparticles have promising potential for the modification and improvement of traditional scaffolds to obtain excellent biocompatibility, tunable stiffness, physical sensing, and stimulus-response capabilities. In the present study, we report magnetic poly(lactic- co-glycolic acid)/polycaprolactone (PLGA/PCL) scaffolds that were fabricated using a combination of the electrospinning technique and layer-by-layer assembly of superparamagnetic iron oxide nanoparticles (IONPs). PLGA/PCL scaffolds assembled with gold nanoparticles were prepared using the same method for comparison. The results showed that the assembled film of nanoparticles on the surface greatly enhanced the hydrophilicity and increased the elastic modulus of the scaffold, which subsequently improved the osteogenesis of the stem cells. Furthermore, the magnetic property of the IONPs proved to be the key factor in enhancing osteogenic differentiation, which explained the superior osteogenic capacity of the magnetic scaffolds compared with that of the gold nanoparticle-assembled scaffold. These results demonstrated the importance of magnetic nanomaterials as a bioactive interface between cells and scaffolds and will promote the design of biomaterials to improve tissue engineering and regenerative medicine efficacy.
在组织工程和再生医学中，一个关键因素是优化种子细胞与支架之间的相互作用，以使细胞能够在自然仿生条件下生长。它们与生物大分子的相似性和许多独特的性质意味着功能性纳米粒子在修饰和改进传统支架以获得优异的生物相容性、可调的硬度、物理传感和刺激响应能力方面具有很大的潜力。在本研究中，我们报告了使用电纺技术和超顺磁性氧化铁纳米粒子（IONP）的层层组装相结合制备的磁性聚（乳酸-共- 乙醇酸）/聚己内酯（PLGA/PCL）支架。使用相同的方法制备了组装有金纳米粒子的 PLGA/PCL 支架进行比较。结果表明，表面上组装的纳米粒子薄膜大大提高了支架的亲水性并增加了其弹性模量，从而促进了干细胞的成骨作用。此外，IONP 的磁性被证明是增强成骨分化的关键因素，这解释了磁性支架比金纳米粒子组装的支架具有更好的成骨能力。这些结果表明了磁性纳米材料作为细胞和支架之间的生物活性界面的重要性，并将促进设计生物材料以提高组织工程和再生医学的效果。
