Department of Respiratory Diseases, The General Hospital of Western Theater Command, Chengdu, Sichuan 610083, P.R. China.
Department of Central Laboratory, The General Hospital of Western Theater Command, Chengdu, Sichuan 610083, P.R. China.
Oncol Rep. 2019 Mar;41(3):1779-1788. doi: 10.3892/or.2019.6976. Epub 2019 Jan 22.
Gallic acid (3,4,5‑trihydroxybenzoic acid; GA), a plant‑derived natural phenolic compound, has been reported to prevent the development and progression of various types of cancers. However, there has been little elaboration of the anticancer effects and underlying mechanisms of GA alone and/or in combination with cisplatin in non‑small cell lung cancer (NSCLC). The aim of the present study was to investigate the anticancer effects of GA on NSCLC A549 cells and its auxiliary effects on the anticancer activity of cisplatin. The results revealed that GA inhibited the proliferation and induced the apoptosis of NSCLC A549 cells in dose‑ and time‑dependent manners, which was associated with upregulated B‑cell lymphoma 2 (Bcl‑2)‑associated X protein (Bax) and downregulated Bcl‑2. Notably, the results also indicated that GA enhanced the anticancer effects of cisplatin in the inhibition of cancer cell proliferation and the induction of cell apoptosis following elevated Bax expression and suppressed Bcl‑2 expression. Furthermore, the results of the present study also demonstrated that GA exerted independent anticancer effects on NSCLC A549 cells, and facilitated the anticancer effects of cisplatin by modulating the JAK/STAT3 signaling pathway and downstream apoptotic molecules. These results may serve as a rationale for further basic studies and preclinical investigations on the anticancer effects of GA and its auxiliary effects on cisplatin function in human NSCLC.
没食子酸（3,4,5-三羟基苯甲酸；GA）是一种植物源性天然酚类化合物，据报道可预防多种类型癌症的发生和发展。然而，对于 GA 单独使用和/或与顺铂联合使用在非小细胞肺癌（NSCLC）中的抗癌作用及其潜在机制，鲜有阐述。本研究旨在探讨 GA 对 NSCLC A549 细胞的抗癌作用及其对顺铂抗癌活性的辅助作用。结果显示，GA 呈剂量和时间依赖性抑制 NSCLC A549 细胞增殖并诱导其凋亡，这与 B 细胞淋巴瘤 2（Bcl-2）相关 X 蛋白（Bax）上调和 Bcl-2 下调有关。值得注意的是，结果还表明，GA 通过上调 Bax 表达和下调 Bcl-2 表达，增强了顺铂抑制癌细胞增殖和诱导细胞凋亡的抗癌作用。此外，本研究结果还表明，GA 对 NSCLC A549 细胞具有独立的抗癌作用，并通过调节 JAK/STAT3 信号通路和下游凋亡分子促进顺铂的抗癌作用。这些结果可能为进一步研究 GA 的抗癌作用及其对顺铂在人类 NSCLC 中功能的辅助作用的基础研究和临床前研究提供依据。
