英国一只狗携带 blaNDM-5、blaCMY-42 和 blaTEM-190 的大肠杆菌 ST410 的发生和特征。
Occurrence and characterization of Escherichia coli ST410 co-harbouring blaNDM-5, blaCMY-42 and blaTEM-190 in a dog from the UK.
机构信息
Axiom Veterinary Laboratories Ltd, The Manor House, Brunel Road, Newton Abbot, UK.
Modernising Medical Microbiology Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK.
出版信息
J Antimicrob Chemother. 2019 May 1;74(5):1207-1211. doi: 10.1093/jac/dkz017.
BACKGROUND/OBJECTIVES: Carbapenemase-producing Enterobacteriaceae (CPE) are a public health threat, and have been found in humans, animals and the environment. Carbapenems are not authorized for use in EU or UK companion animals, and the prevalence of carbapenem-resistant Gram-negative bacilli (CRGNB) in this population is unknown.
METHODS
We investigated CRGNB isolated from animal specimens received by one diagnostic laboratory from 34 UK veterinary practices (September 2015-December 2016). Any Gram-negative isolates from clinical specimens showing reduced susceptibility to fluoroquinolones and/or aminoglycosides and/or cephalosporins were investigated phenotypically and genotypically for carbapenemases. A complete genome assembly (Illumina/Nanopore) was generated for the single isolate identified to investigate the genetic context for carbapenem resistance.
RESULTS
One ST410 Escherichia coli isolate [(CARB35); 1/191, 0.5%], cultured from a wound in a springer spaniel, harboured a known carbapenem resistance gene (blaNDM-5). The gene was located in the chromosome on an integrated 100 kb IncF plasmid, also harbouring other drug resistance genes (mrx, sul1, ant1 and dfrA). The isolate also contained blaCMY-42 and blaTEM-190 on two separate plasmids (IncI1 and IncFII, respectively) that showed homology with other publicly available plasmid sequences from Italy and Myanmar.
CONCLUSIONS
Even though the use of carbapenems in companion animals is restricted, the concurrent presence of blaCMY-42 and other antimicrobial resistance genes could lead to co-selection of carbapenemase genes in this population. Further studies investigating the selection and flow of plasmids carrying important resistance genes amongst humans and companion animals are needed.
背景/目的：产碳青霉烯酶肠杆菌科（CPE）对公共健康构成威胁，已在人类、动物和环境中发现。碳青霉烯类药物未被授权用于欧盟或英国的伴侣动物，因此，该人群中耐碳青霉烯类革兰氏阴性菌（CRGNB）的流行情况尚不清楚。
方法
我们调查了一家诊断实验室从 34 家英国兽医诊所（2015 年 9 月至 2016 年 12 月）收到的动物标本中分离出的 CRGNB。对任何从临床标本中分离出的对氟喹诺酮类和/或氨基糖苷类和/或头孢菌素类药物敏感性降低的革兰氏阴性菌进行表型和基因型检测以确定碳青霉烯酶。对鉴定出的单一分离株进行全基因组组装（Illumina/Nanopore），以研究碳青霉烯类耐药的遗传背景。
结果
从一只弹跳猎犬的伤口中培养出一株 ST410 大肠杆菌（CARB35），其对碳青霉烯类药物的耐药性为 1/191（0.5%），携带已知的碳青霉烯类耐药基因（blaNDM-5）。该基因位于染色体上一个整合的 100kb IncF 质粒上，该质粒还携带其他耐药基因（mrx、sul1、ant1 和 dfrA）。该分离株还含有 blaCMY-42 和 blaTEM-190 两个位于不同质粒（IncI1 和 IncFII）上的基因，与来自意大利和缅甸的其他公开可用的质粒序列具有同源性。
结论
尽管在伴侣动物中使用碳青霉烯类药物受到限制，但 blaCMY-42 和其他抗菌药物耐药基因的同时存在可能导致该人群中碳青霉烯酶基因的共同选择。需要进一步研究在人类和伴侣动物中携带重要耐药基因的质粒的选择和流动。
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