Li K, Yang Q H, Zhi H J, Gai J Y
Soybean Research Institute of Nanjing Agricultural University; National Center for Soybean Improvement; National Key Laboratory of Crop Genetics and Germplasm Enhancement, Weigang 1, Nanjing 210095, China.
Plant Dis. 2010 Mar;94(3):351-357. doi: 10.1094/PDIS-94-3-0351.
From 1,417 leaf samples with Soybean mosaic virus (SMV)-like symptoms collected from about 1,500 fields of 117 counties of 13 provinces in Southern China during 2004 to 2006, 201 SMV isolates were obtained after biological purification with single-lesion isolation and serological determination. Based on disease reactions on 10 differentials used in China as a representative from various reported differential cultivar systems, the isolates were grouped into 12 strains. Among them, eight were the same as those previously reported from Northeast China and the Huang-Huai Valleys while the other four were new and were designated as SC18, SC19, SC20, and SC21. Strains SC15 and SC18, which were predominant and widespread in Southern China, accounted for 32.3 and 26.4% of the total isolates and were distributed in 9 and 10 provinces, respectively. Thus far, 21 strains have been identified in the three soybean-producing regions in China under the uniform differentials system. According to their responses on the 10 differentials, the strains were clustered into five groups at similarity coefficient 0.6. Among them, SC15 in cluster V is the most virulent strain because it infects all differentials. Therefore, specific attention should be placed on control of its prevalence in soybean production.
2004年至2006年期间，从中国南方13个省份117个县约1500块田地采集的1417份具有大豆花叶病毒（SMV）样症状的叶片样本中，通过单斑分离和血清学测定进行生物学纯化后，获得了201株SMV分离物。根据在中国使用的10个鉴别品种（作为来自各种报道的鉴别品种系统的代表）上的病害反应，这些分离物被分为12个株系。其中，8个与先前在中国东北和黄淮流域报道的株系相同，而另外4个是新的，分别命名为SC18、SC19、SC20和SC21。在中国南方占主导地位且分布广泛的SC15和SC18株系，分别占分离物总数的32.3%和26.4%，分别分布在9个和10个省份。到目前为止，在中国三个大豆产区，在统一的鉴别品种系统下已鉴定出21个株系。根据它们在10个鉴别品种上的反应，这些株系在相似系数为0.6时被聚类为五组。其中，聚类V中的SC15是最具毒性的株系，因为它能感染所有鉴别品种。因此，在大豆生产中应特别关注控制其流行。
